Isolation of a serum component that stimulates the spreading of cells in culture.

The Biochemical journal ·Vol. 185 ·No. 2 ·1980-02-01 ·Pages 349-54

Whateley JG, Knox P

Abstract

A foetal-calf-serum component has been isolated that stimulates cells in culture to adopt typical fibroblast morphology. This "spreading" factor is a glycoprotein with a mol. wt. of approx. 62000 and contains 12% carbohydrate; it has an isoelectric point of 4.8. The method described allows purification to homogeneity as judged by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and analytical isoelectric focusing. Under cell-culture conditions the factor is active at 18 micrograms/ml.

MeSH Terms

Amino Acids/analysis Animals Cattle Cell Line Cricetinae Culture Media/analysis Dialysis Dose-Response Relationship, Drug Electrophoresis Fetal Blood/analysis Fibroblasts/cytology,drug effects Glycoproteins/blood,isolation & purification,pharmacology Isoelectric Focusing Kidney/cytology,drug effects Vitronectin

Chemicals

Amino Acids Culture Media Glycoproteins Vitronectin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Whateley J G

Knox P

References (18)

18 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1850349

Published

1980-02-01

Pages

349-54

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1161360

Subset

External Links

PubMed source DOI: 10.1042/bj1850349 PMC Full Text View journal details

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Isolation of a serum component that stimulates the spreading of cells in culture.

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