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PMID: 6156981 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Subclass restriction of murine antibodies. III. Antigens that stimulate IgG3 in mice stimulate IgG2c in rats.

The Journal of experimental medicine ·Vol. 152 ·No. 1 ·1980-07-01 ·Pages 209-18

Der Balian GP, Slack J, Clevinger BL, Bazin H, Davie JM

Abstract

The IgG subclass distribution of rat antibodies to 13 different antigens was measured. Antibodies to protein and hapten-protein conjugates were predominantly IgG2a. Antigens labeled thymus-independent type 1, based upon responses in mice, stimulated both IgG2b and IgG2c antibodies, but little IgG2a. Polysaccharide and hapten-polysaccharide antigens (thymus-independent type 2) as well as phosphocholine-keyhole limpet hemocyanin, stimulated predominantly IgG2c antibodies. A division of antigens into essentially the same categories has been made on the basis of subclass restriction in mice. Antigens that stimulate IgG2c in rats stimulate IgG3 in mice. Thus, by comparing subclass preference with a variety of antigens, functional analogues among subclasses in different species can be identified.

MeSH Terms
Animals Antibody Formation Antigens/immunology B-Lymphocytes/immunology Epitopes Female Immunization Immunoglobulin Allotypes/biosynthesis Immunoglobulin G/biosynthesis,immunology Male Mice/immunology Rats/immunology
Chemicals
Antigens Epitopes Immunoglobulin Allotypes Immunoglobulin G
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Der Balian G P
Slack J
Clevinger B L
Bazin H
Davie J M
References (36)
36 references, click to expand
  1. Studies on the effect of the carrier molecule on antihapten antibody synthesis. I. Effect of carrier on the nature of the antibody synthesized.
    J Exp Med. 1966 Apr 1;123(4):673-88 PMID: 4160398
  2. The use of a wate-soluble carbodiimide as a coupling reagent in the passive hemagglutination test.
    J Immunol. 1966 Dec;97(6):791-6 PMID: 4163006
  3. Studies on human antibodies. VI. Selective variations in subgroup composition and genetic markers.
    J Exp Med. 1968 Mar 1;127(3):633-46 PMID: 4169968
  4. Antitrinitrophenyl (TNP) plaque assay. Primary response of Balb/c mice to soluble and particulate immunogen.
    Proc Soc Exp Biol Med. 1969 Nov;132(2):575-81 PMID: 5355110
  5. A new mouse immunoglobulin: IgG3.
    J Exp Med. 1971 Feb 1;133(2):289-304 PMID: 5133863
  6. Affinity labeling of a phosphorylcholine binding mouse myeloma protein.
    Biochemistry. 1972 Feb 29;11(5):766-71 PMID: 5059886
  7. Antigenic determinants on immunoglobulins of nonhuman primates.
    Transplant Proc. 1972 Mar;4(1):129-35 PMID: 4110958
  8. Adsorbents for affinity chromatography. Use of N-hydroxysuccinimide esters of agarose.
    Biochemistry. 1972 Jun 6;11(12):2291-9 PMID: 5028497
  9. Cloning of mouse myeloma cells and detection of rare variants.
    J Cell Physiol. 1972 Jun;79(3):429-40 PMID: 4625122
  10. Streptococcal group A carbohydrate antibodies in mice: evidence for strain differences in magnitude and restriction of the response, and for thymus dependence.
    Eur J Immunol. 1972 Apr;2(2):138-43 PMID: 4118771
  11. Effect of carrier priming on the distribution of anti-hapten antibodies between IgG1 and IgG2 isotypes in the hyperimmune guinea pig.
    J Immunol. 1974 Aug;113(2):677-87 PMID: 4136042
  12. Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c.
    Eur J Immunol. 1974 Jan;4(1):44-8 PMID: 4137315
  13. Stimulation of a T-independent primary anti-hapten response in vitro by TNP-lipopolysaccharide (TNP-LPS).
    J Immunol. 1975 Jan;114(1 Pt 2):360-4 PMID: 1090653
  14. Thymus-independent antigens: the preparation of covalent, hapten-ficoll conjugates.
    J Immunol. 1975 Feb;114(2 Pt 1):704-9 PMID: 1079035
  15. DNP-Lys-ficoll: a T-independent antigen which elicits both IgM and IgG anti-DNP antibody-secreting cells.
    J Immunol. 1975 May;114(5):1585-9 PMID: 1091706
  16. Clonal dominance. I. Restricted nature of the IgM antibody response to group A streptococcal carbohydrate in mice.
    J Exp Med. 1975 Jun 1;141(6):1291-1307 PMID: 1092797
  17. A plaque assay for all cells secreting Ig of a given type or class.
    Eur J Immunol. 1976 Aug;6(8):588-90 PMID: 789096
  18. In vitro responses of CBA/N mice: spleen cells of mice with an X-linked defect that precludes immune responses to several thymus-independent antigens can respond to TNP-lipopolysaccharide.
    J Immunol. 1976 Oct;117(4):1363-9 PMID: 789772
  19. Identification of a B-cell surface structure involved in antigen-dependent triggering: absence of this structure on B cells from CBA/N mutant mice.
    J Exp Med. 1977 Jan 1;145(1):10-20 PMID: 299766
  20. B-lymphocyte heterogeneity: development and characterization of an alloantiserum which distinguishes B-lymphocyte differentiation alloantigens.
    J Exp Med. 1977 Jan 1;145(1):101-10 PMID: 299767
  21. Antibodies to major histocompatibility antigens produced by hybrid cell lines.
    Nature. 1977 Apr 7;266(5602):550-2 PMID: 558524
  22. Functional, structural, and antigenic similarities of guinea pig anti-phosphorylcholine antibodies.
    J Immunol. 1977 Jun;118(6):1987-94 PMID: 325132
  23. Complete sharing of light chain spectrotypes by murine IgM and IgG anti-streptococcal antibodies.
    J Immunol. 1977 Jun;118(6):2161-6 PMID: 405425
  24. The ontogeny of thymic independent antibody responses in vitro in normal mice and mice with an X-linked B cell defect.
    J Immunol. 1977 Dec;119(6):1874-8 PMID: 334975
  25. Surface immunoglobulin D as a functional receptor for a subclass of B lymphocytes.
    Immunol Rev. 1977;37:89-104 PMID: 338469
  26. Subclass restriction of murine anti-carbohydrate antibodies.
    J Immunol. 1978 Aug;121(2):566-72 PMID: 79606
  27. T-independent responses in B cell-defective CBA/N mice to Brucella abortus and to trinitrophenyl (TNP) conjugates of Brucella abortus.
    Eur J Immunol. 1978 Jul;8(7):459-63 PMID: 99320
  28. A better cell line for making hybridomas secreting specific antibodies.
    Nature. 1978 Nov 16;276(5685):269-70 PMID: 714156
  29. Immunoglobulin subclass-specific immunodeficiency in mice with an X-linked B-lymphocyte defect.
    J Exp Med. 1979 Apr 1;149(4):993-8 PMID: 311819
  30. Ontogeny of murine B-cell responses to thymus-independent trinitrophenyl antigens.
    Cell Immunol. 1979 May;44(2):367-80 PMID: 313267
  31. Expression of a cross reactive idiotype on the IgG2c subclass of rat anti-streptococcal carbohydrate antibody.
    Mol Immunol. 1979 Apr;16(4):281-5 PMID: 386080
  32. Isotypes of IgG: comparison of the primary structures of three pairs of isotypes which differ in their ability to activate complement.
    Mol Immunol. 1979 Nov;16(11):907-17 PMID: 118918
  33. Subclass restriction of murine antibodies. II. The IgG plaque-forming cell response to thymus-independent type 1 and type 2 antigens in normal mice and mice expressing an X-linked immunodeficiency.
    J Exp Med. 1980 Apr 1;151(4):853-62 PMID: 6966310
  34. B-cell tolerance. II. Trinitrophenyl human gamma globulin-induced tolerance in adult and neonatal murine B cells responsive to thymus-dependent and independent forms of the same hapten.
    J Exp Med. 1977 Mar 1;145(3):778-83 PMID: 95788
  35. Properties of guinea pig 7S antibodies. I. Electrophoretic separation of two types of guinea pig 7S antibodies.
    J Exp Med. 1963 Jun 1;117:937-49 PMID: 13967257
  36. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.
    Biochem J. 1963 Oct;89:114-23 PMID: 14097352
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.152.1.209
Published
1980-07-01
Pages
209-18
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2185890
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.152.1.209 PMC Full Text View journal details
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