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PMID: 6157773 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Presence of highly conserved idiotypic determinants in a family of antibodies that constitute an intrastrain cross-reactive idiotype.

The Journal of experimental medicine ·Vol. 152 ·No. 3 ·1980-09-01 ·Pages 703-11

Lamoyi E, Estess P, Capra JD, Nisonoff A

Abstract

It has been shown that A/J anti-p-azophenylarsonate antibodies that share a major cross-reactive idiotype (CRI) comprise a family of closely related, but nonidentical, molecules. Our results demonstrate that 12 of 14 monoclonal hybridoma products that express the CRI have in common at least one highly conserved idiotypic determinant. It is proposed that this reflects conservation of a portion of the amino acid sequence, presumably in hypervariable regions. That the conserved determinant(s0 are located in the region of the hapten-binding site is indicated by the ability of haptens to inhibit idiotype-anti-idiotype interactions involving the conserved, or public determinants.

MeSH Terms
Animals Antibody Diversity Azo Compounds/immunology Clone Cells/immunology Cross Reactions Epitopes Haptens Immunoglobulin Idiotypes Ligands Mice Mice, Inbred A p-Azobenzenearsonate/immunology
Chemicals
Azo Compounds Epitopes Haptens Immunoglobulin Idiotypes Ligands p-Azobenzenearsonate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lamoyi E
Estess P
Capra J D
Nisonoff A
References (24)
24 references, click to expand
  1. Antigenic specificities related to the cold agglutinin activity of gamma M globulins.
    Science. 1968 Jul 26;161(3839):379-81 PMID: 4173573
  2. Quantitative investigations of idiotypic antibodies. VI. Idiotypic specificity as a potential genetic marker for the variable regions of mouse immunoglobulin polypeptide chains.
    J Exp Med. 1972 Mar 1;135(3):579-95 PMID: 4110016
  3. Suppression of idiotypic specificities in adult mice by administration of antiidiotypic antibody.
    J Exp Med. 1972 Jun 1;135(6):1293-300 PMID: 4623607
  4. Immunoglobulin-producing tumors and myeloma proteins of mice.
    Physiol Rev. 1972 Jul;52(3):631-719 PMID: 4555516
  5. Distribution of a cross-reactive idiotypic specificity in inbred strains of mice.
    J Exp Med. 1973 Apr 1;137(4):855-69 PMID: 4120895
  6. Immunochemical analysis of the idiotypes of mouse myeloma proteins with specificity for levan or dextran.
    J Exp Med. 1974 Jan 1;139(1):137-47 PMID: 4128444
  7. Clonal nature of the immune response to phosphorylcholine. IV. Idiotypic uniformity of binding site-associated antigenic determinants among mouse antiphosphorylcholine antibodies.
    J Exp Med. 1974 Sep 1;140(3):673-86 PMID: 4137581
  8. Multiple individual and cross-specific indiotypes on 13 levan-binding myeloma proteins of BALB/c mice.
    J Exp Med. 1975 Jul 1;142(1):106-19 PMID: 1151286
  9. Assessing B cell diversification by antigen receptor and precursor cell analysis.
    Ann Immunol (Paris). 1976 Jun-Jul;127(3-4):489-502 PMID: 60906
  10. Analysis of V gene expression in the immune response by cell fusion.
    Curr Top Microbiol Immunol. 1978;81:20-6 PMID: 567558
  11. A better cell line for making hybridomas secreting specific antibodies.
    Nature. 1978 Nov 16;276(5685):269-70 PMID: 714156
  12. Idiotypic analysis of monoclonal antibodies to poly(Glu60Ala30Tyr10).
    Proc Natl Acad Sci U S A. 1979 Jun;76(6):2942-6 PMID: 288079
  13. Suppression of idiotype and generation of suppressor T cells with idiotype-conjugated thymocytes.
    J Exp Med. 1979 Oct 1;150(4):909-18 PMID: 159936
  14. The cross-reactive idiotype of anti-4-azobenzenearsonate hybridoma-derived antibodies in A/J mice constitutes multiple heavy chains.
    Hoppe Seylers Z Physiol Chem. 1980;361(2):191-5 PMID: 6153633
  15. Hybridoma proteins expressing the predominant idiotype of the antiazophenylarsonate response of A/J mice.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):1120-4 PMID: 6767242
  16. Structural studies on induced antibodies with defined idiotypic specificities--VIII. NH2-terminal amino acid sequence analysis of the heavy and light chain variable regions of monoclonal anti-para-azophenylarsonate antibodies from A/J mice differing with respect to a cross-reactive idiotype.
    Mol Immunol. 1979 Dec;16(12):1111-8 PMID: 120496
  17. Heterogeneity of an intrastrain cross-reactive idiotype associated with anti-p-azophenylarsonate antibodies of A/J mice.
    J Immunol. 1980 Jun;124(6):2834-40 PMID: 7373052
  18. Structural studies on induced antibodies with defined idiotypic specificities. IX. Framework differences in the heavy- and light-chain-variable regions of monoclonal anti-p-azophenylarsonate antibodies from A/J mice differing with respect to a cross-reactive idiotype.
    J Exp Med. 1980 Apr 1;151(4):863-75 PMID: 6768828
  19. Analysis of the repertoire of anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibodies in C 57 BL/6 mice by cell fusion. II. Characterization of idiotopes by monoclonal anti-idiotope antibodies.
    Eur J Immunol. 1979 Dec;9(12):1004-13 PMID: 398773
  20. Azoproteins. I. Spectrophotometric studies of amino acid azo derivatives.
    J Biol Chem. 1959 Jul;234(7):1726-30 PMID: 13672953
  21. SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS.
    Science. 1964 Aug 14;145(3633):709-10 PMID: 14168277
  22. Standardization of the chloramine-T method of protein iodination.
    Proc Soc Exp Biol Med. 1970 Mar;133(3):989-92 PMID: 4190944
  23. Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.
    J Biol Chem. 1970 Jun;245(12):3059-65 PMID: 5432796
  24. Quantitative investigations of idiotypic antibodies. IV. Inhibition by specific haptens of the reaction of anti-hapten antibody with its anti-idiotypic antibody.
    J Exp Med. 1970 Nov;132(5):951-62 PMID: 4097134
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.152.3.703
Published
1980-09-01
Pages
703-11
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2185919
Subset
IM
Grants
NIAID NIH HHS · AI-12127 · United States
NIAID NIH HHS · AI-12895 · United States
NIAID NIH HHS · AI-12907 · United States
External Links
PubMed source DOI: 10.1084/jem.152.3.703 PMC Full Text View journal details
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