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PMID: 6160370 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

DNA polymerase III-dependent repair synthesis in response to bleomycin in toluene-treated Escherichia coli.

Molecular & general genetics : MGG ·Vol. 179 ·No. 3 ·1980-00-00 ·Pages 595-605

Ross SL, Sharma S, Moses RE

Abstract

Bleomycin (BLM) is an antitumor drug which interacts with and damages DNA. We have reported a repair response dependent on DNA polymerase I in toluene-treated Escherichia coli. We report here that DNA polymerase III can also catalyze a repair response in toluene-treated E. coli following exposure to BLM. Polymerase III-mediated synthesis differs because it is ATP-dependent, whereas polymerase I-mediated repair synthesis is not. Polymerase III repair synthesis is independent of replicative synthesis, as demonstrated in a polA-, dnaBts strain, or use of Novobiocin to inhibit replication, and replication persists in the presence of repair synthesis. It appears that ATP-dependent repair synthesis in response to BLM is also present in polA+ strains. Repair synthesis does not require the uvrA gene product.

MeSH Terms
Bleomycin/pharmacology DNA Polymerase III/genetics,metabolism DNA Repair/drug effects DNA Replication/drug effects DNA, Bacterial/genetics DNA-Directed DNA Polymerase/metabolism Escherichia coli/drug effects,genetics Toluene/pharmacology
Chemicals
DNA, Bacterial Bleomycin Toluene DNA Polymerase III DNA-Directed DNA Polymerase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ross S L
Sharma S
Moses R E
References (42)
42 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00271750
Published
1980-00-00
Pages
595-605
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM-19122 · United States
NIGMS NIH HHS · GM-24711 · United States
External Links
PubMed source DOI: 10.1007/BF00271750
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