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PMID: 6161374 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of the amyloid-related apoprotein (SAA) from human high density lipoprotein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 11 ·1980-11-00 ·Pages 6860-4

Eriksen N, Benditt EP

Abstract

Two apoproteins immunologically related to the 9000-dalton abnormal tissue constituent known as amyloid protein AA were isolated from the lipoprotein density interval 1.125-1.21 g/cm3 (HDL3) of a pool of human serums by delipidation, gel filtration, and ion-exchange chromatography. Lesser amounts of the same apoproteins were isolated from the density interval 1.063-1.125 g/cm3 (HDL2). These apoproteins, designated apoSAA1 and apoSAA2, have molecular weights near 11,500, almost identical amino acid compositions, and slightly different isoelectric points. Their amino acid sequences are identical as far as determined (30 residues), except that apoSAA2 lacks the NH2-terminal arginine found in apoSAA1. The sequence is homologous with that of amyloid protein AA, which thus has residing in the plasma high density lipoproteins a potential precursor whose biological significance and function remain to be determined.

MeSH Terms
Amino Acid Sequence Amino Acids/analysis Amyloid/analysis Apolipoproteins/analysis Epitopes Humans Isoelectric Point Lipoproteins, HDL/analysis Molecular Weight Serum Amyloid A Protein/analysis,immunology
Chemicals
Amino Acids Amyloid Apolipoproteins Epitopes Lipoproteins, HDL Serum Amyloid A Protein
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Eriksen N
Benditt E P
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43 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.11.6860
Published
1980-11-00
Pages
6860-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC350390
Subset
IM
Grants
NIGMS NIH HHS · GM-27335 · United States
NHLBI NIH HHS · HL-03174 · United States
External Links
PubMed source DOI: 10.1073/pnas.77.11.6860 PMC Full Text View journal details
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