The isolation of cloned cDNA sequences which are differentially expressed in human lymphocytes and fibroblasts.
Poly(A)+ RNA populations derived from normal lymphocytes and fibroblasts have been compared by hybridising each RNA to cDNA derived from the other RNA population. This indicated that approximately 75% of the sequences were common to both, and that these were present at different concentrations in the two cell types. The two RNA populations were further compared by hybridising them to a cDNA recombinant library derived from lymphocyte poly(A)+ RNA. This allowed the identification of clones containing sequences which are abundant in lymphocyte poly(A)+ RNA but absent or rare in fibroblast poly(A)+ RNA. A direct estimation of the abundance of five of these sequences in lymphocyte cDNA demonstrated that clones can be detected by such a procedure if they represent 0.2% or greater of the original cDNA population.
16 references, click to expand
-
A membrane-filter technique for the detection of complementary DNA.Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
-
A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.Proc Natl Acad Sci U S A. 1967 May;57(5):1514-21 PMID: 5231757
-
Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
-
Tissue typing using a routine one-step lymphocyte separation procedure.Br J Haematol. 1970 Feb;18(2):229-35 PMID: 5439527
-
Kinetic complexity of RNA molecules.J Mol Biol. 1972 Jan 14;63(1):21-39 PMID: 4622766
-
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
-
Reversal of bacteriophage T4 induced polynucleotide kinase action.Biochemistry. 1973 Dec 4;12(25):5050-5 PMID: 4366306
-
A kinetic estimation of base sequence complexity of nuclear poly(A)-containing RNA in mouse Friend cells.Cell. 1975 Feb;4(2):121-9 PMID: 1168544
-
Detection of specific sequences among DNA fragments separated by gel electrophoresis.J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
-
Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
-
Messenger RNA for myosin polypeptides: isolation from single myogenic cell cultures.Cell. 1977 Feb;10(2):265-73 PMID: 837449
-
The expression of three abundance classes of messenger RNA in mouse tissues.Cell. 1976 Dec;9(4 PT 2):761-74 PMID: 1017013
-
Analysis of the genetic complexity and abundance classes of messenger RNA in human liver and leukemic cells.Biochim Biophys Acta. 1979 Mar 28;562(1):92-102 PMID: 86365
-
Use of a cDNA library for studies on evolution and developmental expression of the chorion multigene families.Cell. 1979 Dec;18(4):1303-16 PMID: 519770
-
Filter replicas and permanent collections of recombinant DNA plasmids.Nucleic Acids Res. 1979 Dec 20;7(8):2115-36 PMID: 118435
-
Use of a cloned library for the study of abundant poly(A)+RNA during Xenopus laevis development.Dev Biol. 1980 May;76(2):449-64 PMID: 7390013
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