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PMID: 6162788 Published · ppublish English Journal Article

Dissociation of H-2 recognition by antibody and cytotoxic T cells of a cloned murine leukemia cell line.

Immunogenetics ·Vol. 12 ·No. 1-2 ·1981-00-00 ·Pages 101-15

Portis JL, McAtee FJ

Abstract

The differential expression of H-2 specificities recognized by antibody and by cytotoxic T lymphocytes (CTL) has been studied using a clone (FY7) of the C57BL/6 leukemia cell line FBL-3 (H-2b/H-2b). Unlike C57BL/10 spleen cells, EL-4 lymphoma cells and Y57-2C leukemia cells (all H-2b/H-2b), FY7 failed to induce the primary in vitro generation of anti-H-2b CTL by (B10.A x A)F1 (H-2a/H-2a) or B10.D2 x BALB/c)F1 (H-2d/H-2d) responder spleen cells. In addition, FY7 was not lysed by, and did not competitively inhibit anti-H-2b CTL. Quantitative absorption tests with H-2Kb and H-2Db antisera revealed that FY7 expressed these antigens in quantitatively similar amounts to EL-4. The H-2Kb product of FY7 appeared to be identical with that of C57BL/10 spleen cells both in apparent molecular weight and isoelectric point. Yet FY7 failed to inhibit anti-H-2Kb CTL competitively in a cold target inhibition assay. Possible mechanisms are discussed for the lack of T-lymphocyte recognition of the H-2Kb-gene product expressed by FY7.

MeSH Terms
Absorption Animals Antibody Specificity Antigens, Surface/immunology Cell Line Clone Cells/immunology Cytotoxicity, Immunologic Epitopes H-2 Antigens/immunology Immunologic Techniques Leukemia, Experimental/immunology Lymphocyte Activation Male Mice Mice, Inbred C57BL T-Lymphocytes/immunology
Chemicals
Antigens, Surface Epitopes H-2 Antigens
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Portis J L
McAtee F J
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38 references, click to expand
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Article Info
Journal
Immunogenetics
Abbr.
Immunogenetics
ISSN
0093-7711
DOI
10.1007/BF01561654
Published
1981-00-00
Pages
101-15
Language
English
Region
United States
NLM ID
0420404
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01561654 View journal details
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