Home LiteratureArticle Details
PMID: 6163693 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Biochemical and immunological approaches to the study of gap junctional communication.

In vitro ·Vol. 16 ·No. 12 ·1980-12-00 ·Pages 1057-67

Hertzberg EL

Abstract

Studies on gap junctions isolated from rat liver by a procedure that avoids exogenous proteolysis (Hertzberg, E. L.; Gilula. N. B.; J. Biol. Chem. 254: 2138-2147; 1979) are described. The original isolation procedure was modified to increase the yield and has been extended to the preparation of gap junctions from mouse and bovine liver. Peptide map studies showed that the 27,000-dalton polypeptides present in liver gap junction preparations from all three sources are homologous and are not derived from other polypeptides of higher molecular weight that are observed in cruder preparations. Similar studies with lens fiber junctions demonstrated no homology between liver and lens junction polypeptides. Antibodies to the lens junction polypeptides did not cross-react with the liver gap junction polypeptide, further supporting this conclusion.

MeSH Terms
Animals Antibody Specificity Cattle Cell Communication Cross Reactions Crystallins/immunology Epitopes Intercellular Junctions/immunology,metabolism Liver/immunology Membrane Proteins/immunology Mice Rats Species Specificity
Chemicals
Crystallins Epitopes Membrane Proteins
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Hertzberg E L
References (32)
32 references, click to expand
  1. Changes in electrical properties of rat myometrium during gestation and following hormonal treatments.
    J Physiol. 1976 Sep;260(2):315-33 PMID: 978524
  2. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
    J Biol Chem. 1977 Feb 10;252(3):1102-6 PMID: 320200
  3. Independent lines of evidence suggesting a major gap junctional protein with a molecular weight of 26,000.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):970-4 PMID: 6928694
  4. Lens gap junctions: a structural hypothesis for nonregulated low-resistance intercellular pathways.
    Invest Ophthalmol Vis Sci. 1979 Nov;18(11):1104-22 PMID: 511455
  5. Peptide mapping of heterogeneous protein samples.
    J Biol Chem. 1979 Apr 25;254(8):2565-7 PMID: 429300
  6. Lens membranes II. Isolation and characterization of the main intrinsic polypeptide (MIP) of bovine lens fiber membranes.
    Exp Eye Res. 1976 Sep;23(3):365-71 PMID: 976377
  7. Preparation of hepatic gap (communicating) junctions. Identification of the constituent polypeptide subunits.
    Biochem J. 1977 Dec 15;168(3):475-81 PMID: 204288
  8. Fracture faces of frozen membranes.
    Proc Natl Acad Sci U S A. 1966 May;55(5):1048-56 PMID: 5334198
  9. Two forms of isolated gap junctions.
    J Mol Biol. 1979 Dec 5;135(2):451-64 PMID: 537084
  10. A study of communication specificity between cells in culture.
    J Cell Biol. 1977 Dec;75(3):769-87 PMID: 562887
  11. The isolation of mouse hepatocyte gap junctions. Preliminary chemical characterization and x-ray diffraction.
    J Cell Biol. 1972 Sep;54(3):646-56 PMID: 4339819
  12. The protein component of mouse hepatocyte gap junctions.
    FEBS Lett. 1977 Jun 15;78(2):295-9 PMID: 885255
  13. Maturation of the head of bacteriophage T4. I. DNA packaging events.
    J Mol Biol. 1973 Nov 15;80(4):575-99 PMID: 4204102
  14. Junctional intercellular communication and the control of growth.
    Biochim Biophys Acta. 1979 Feb 4;560(1):1-65 PMID: 216404
  15. Cell junctions and intercellular communication.
    In Vitro. 1980 Dec;16(12):1010-7 PMID: 7216231
  16. Voltage dependence of junctional conductance in early amphibian embryos.
    Science. 1979 Apr 27;204(4391):432-4 PMID: 312530
  17. Reconstitution of purified detergent-soluble HLA-A and HLA-B antigens into phospholipid vesicles.
    Proc Natl Acad Sci U S A. 1978 Jul;75(7):3230-4 PMID: 356051
  18. Permeability of the cell-to-cell membrane channels in mammalian cell juncton.
    Science. 1979 Jul 27;205(4404):404-7 PMID: 377490
  19. Gap junction structures. I. Correlated electron microscopy and x-ray diffraction.
    J Cell Biol. 1977 Aug;74(2):605-28 PMID: 885916
  20. The vertebrate eye lens.
    Science. 1977 Jul 8;197(4299):127-38 PMID: 877544
  21. Hexagonal array of subunits in tight junctions separated from isolated rat liver plasma membranes.
    J Cell Biol. 1968 Jul;38(1):15-24 PMID: 5691971
  22. Structure and biochemistry of mouse hepatic gap junctions.
    J Mol Biol. 1979 Aug 5;132(2):193-218 PMID: 537050
  23. Calcium ion produces graded changes in permeability of membrane channels in cell junction.
    Nature. 1977 Jun 16;267(5612):625-7 PMID: 406571
  24. Gap junction structures. II. Analysis of the x-ray diffraction data.
    J Cell Biol. 1977 Aug;74(2):629-45 PMID: 889612
  25. Intracellular pH in early Xenopus embryos: its effect on current flow between blastomeres.
    J Physiol. 1980 Mar;300:489-504 PMID: 6770084
  26. Protein composition of bovine lens cortical fiber cell membranes.
    Exp Eye Res. 1975 Dec;21(6):581-95 PMID: 1204686
  27. The protein components of the gap junction.
    Cold Spring Harb Symp Quant Biol. 1976;40:45-7 PMID: 1065538
  28. Isolation and characterization of gap junctions from rat liver.
    J Biol Chem. 1979 Mar 25;254(6):2138-47 PMID: 422573
  29. Preparation and properties of nexuses and lipid-enriched vesicles from mouse liver plasma membranes.
    Biochem J. 1972 Jul;128(3):691-700 PMID: 4634836
  30. Structure of the junction between communicating cells.
    Nature. 1980 Feb 7;283(5747):545-9 PMID: 7354837
  31. Transmission of hormonal stimulation by cell-to-cell communication.
    Nature. 1978 Apr 6;272(5653):501-6 PMID: 211417
  32. Communication between cells of different type.
    Nature. 1971 Jul 9;232(5306):121-2 PMID: 4933243
Article Info
Journal
In vitro
Abbr.
In Vitro
ISSN
0073-5655
DOI
10.1007/BF02619256
Published
1980-12-00
Pages
1057-67
Language
English
Region
United States
NLM ID
0063733
Subset
IM
Grants
NIMHD NIH HHS · 263-MD-025754 · United States
NIGMS NIH HHS · GM 24753 · United States
NHLBI NIH HHS · HL 16507 · United States
External Links
PubMed source DOI: 10.1007/BF02619256
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2