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PMID: 6164990 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Ribosomal RNA cleavage, nuclease activation and 2-5A(ppp(A2'p)nA) in interferon-treated cells.

Nucleic acids research ·Vol. 9 ·No. 7 ·1981-04-10 ·Pages 1571-81

Wreschner DH, James TC, Silverman RH, Kerr IM

Abstract

Ribosomal RNA (rRNA) in intact ribosomes is cleaved into discrete products on incubation of reticulocyte lysates or L-cell extracts with ppp(A2'p)3A. Cleavage of rRNA may, therefore, provide a useful assay for 2-5A (ppp)A2'p)nA; n = 2 to 4) or for the presence of a 2-5A-dependent nuclease. The results with reticulocyte lysates differed from those obtained in the L-cell-free system in that (a) a different RNA cleavage pattern was produced (with added L-cell ribosomes) and (b) cleavage was fully activated by the analogue ppp(A2'p)3A3'pCp. As might be expected from the relatively high levels of 2-5A present in interferon-treated, encephalomyocarditis virus (EMC)-infected L-cells, rRNA extracted from these cells was also cleaved. The cleavage pattern observed overlapped with that obtained on incubation of an L-cell-free system with 2-5A. Thus, not only is 2-5A present, but the 2-5A-dependent nuclease also appears to be active, in interferon-treated, EMC-infected L-cells.

MeSH Terms

Adenine Nucleotides/metabolism Animals Endonucleases/metabolism Enzyme Activation Interferons/pharmacology Kinetics L Cells/metabolism Mice Oligonucleotides/metabolism Oligoribonucleotides/metabolism RNA, Ribosomal/metabolism Reticulocytes/metabolism

Chemicals

Adenine Nucleotides Oligonucleotides Oligoribonucleotides RNA, Ribosomal 2',5'-oligoadenylate Interferons Endonucleases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Wreschner D H

James T C

Silverman R H

Kerr I M

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21 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/9.7.1571

Published

1981-04-10

Pages

1571-81

Language

English

Region

England

NLM ID

0411011

PMCID

PMC326782

Subset

External Links

PubMed source DOI: 10.1093/nar/9.7.1571 PMC Full Text View journal details

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Ribosomal RNA cleavage, nuclease activation and 2-5A(ppp(A2'p)nA) in interferon-treated cells.

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