Home LiteratureArticle Details
PMID: 6165014 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Partial purification and characterization of human gamma (immune) interferon.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 3 ·1981-03-00 ·Pages 1601-5

Yip YK, Pang RH, Urban C, Vilcek J

Abstract

Human gamma (immune) interferon (IFN-gamma) was produced in lymphocyte cultures stimulated with a phorbol ester (12-O-tetradecanoylphorbol 13-acetate) and purified phytohemagglutinin. Physicochemical analysis showed that human IFN-gamma is a glycoprotein with an isoelectric point around 8.6 and an apparent molecular weight of 58,000 +/- 3000. A purification process for IFN-gamma was developed consisting of sequential chromatographic separations on controlled-pore glass, concanavalin A-Sepharose, and Bio-Gel P-200. This purification process resulted in an increase in specific activity from about 10(4) (crude culture fluid) to an estimated 10(7) units per mg of protein with a cumulative recovery of about 40% of the IFN activity.

MeSH Terms
Chromatography, Affinity Chromatography, Gel Concanavalin A Electrophoresis, Polyacrylamide Gel Humans Interferons/biosynthesis,isolation & purification Lymphocytes/drug effects,metabolism Macromolecular Substances Molecular Weight Tetradecanoylphorbol Acetate/pharmacology
Chemicals
Macromolecular Substances Concanavalin A Interferons Tetradecanoylphorbol Acetate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yip Y K
Pang R H
Urban C
Vilcek J
References (17)
17 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Production, partial purification and characterization of human and murine interferons--type II.
    Mol Immunol. 1980 May;17(5):625-33 PMID: 6163960
  3. Correlation of physicochemical and antigenic properties of human leukocyte interferon subspecies.
    Arch Virol. 1977;55(1-2):121-9 PMID: 72554
  4. Inhibition of murine osteogenic sarcomas by treatment with type I or type II interferon.
    J Natl Cancer Inst. 1978 Sep;61(3):871-4 PMID: 278865
  5. Glycosylation of interferons. Effects of tunicamycin on human immune interferon.
    J Biol Chem. 1978 Nov 10;253(21):7612-5 PMID: 701277
  6. Time and dosage dependence of immunoenhancement by murine type II interferon preparations.
    Cell Immunol. 1978 Oct;40(2):285-93 PMID: 363280
  7. Large-scale production and physicochemical characterization of human immune interferon.
    Infect Immun. 1979 Oct;26(1):36-41 PMID: 40881
  8. Immune and virus-induced interferons may activate cells by different derepressional mechanisms.
    Nature. 1980 Jan 24;283(5745):400-2 PMID: 7352017
  9. Human fibroblast interferon: amino acid analysis and amino terminal amino acid sequence.
    Science. 1980 Feb 1;207(4430):525-6 PMID: 7352259
  10. Amino terminal sequence of the major component of human lymphoblastoid interferon.
    Science. 1980 Feb 1;207(4430):527-8 PMID: 7352260
  11. Human leukocyte and fibroblast interferons are structurally related.
    Nature. 1980 Jun 19;285(5766):547-9 PMID: 6157095
  12. Interferon nomenclature.
    Nature. 1980 Jul 10;286(5769):110 PMID: 6157097
  13. The nucleotide sequence of a cloned human leukocyte interferon cDNA.
    Gene. 1980 Jun;10(1):1-10 PMID: 6157600
  14. The nucleotide sequence of human fibroblast interferon cDNA.
    Gene. 1980 Jun;10(1):11-5 PMID: 6157601
  15. The structure of one of the eight or more distinct chromosomal genes for human interferon-alpha.
    Nature. 1980 Oct 2;287(5781):401-8 PMID: 6159536
  16. A family of structural genes for human lymphoblastoid (leukocyte-type) interferon.
    Nature. 1980 Oct 2;287(5781):408-11 PMID: 6159537
  17. Binding of human fibroblast interferon to concanavalin A-agarose. Involvement of carbohydrate recognition and hydrophobic interaction.
    Biochemistry. 1976 Feb 10;15(3):704-13 PMID: 1252420
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.3.1601
Published
1981-03-00
Pages
1601-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319179
Subset
IM
Grants
NIAID NIH HHS · AI-07057 · United States
NIAID NIH HHS · AI-12948 · United States
NIAID NIH HHS · N01-AI-01269 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.3.1601 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2