Automated synthesis of gene fragments.

Science (New York, N.Y.) ·Vol. 214 ·No. 4518 ·1981-10-16 ·Pages 270-4

Alvarado-Urbina G, Sathe GM, Liu WC, Gillen MF, Duck PD, Bender R, Ogilvie KK

Abstract

The DNA/RNA Synthesizer provides a complete and automated procedure for the synthesis of DNA sequences. Each base unit is added in a 30-minute cycle, permitting a tetradecamer to be constructed in 6 1/2 hours. The complete procedure is described, including a practical procedure for isolation and purification of the desired DNA sequence.

MeSH Terms

Automation Chemical Phenomena Chemistry DNA/chemical synthesis Genes, Synthetic RNA/chemical synthesis Solubility

Chemicals

RNA DNA

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Alvarado-Urbina G

Sathe G M

Liu W C

Gillen M F

Duck P D

Bender R

Ogilvie K K

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.6169150

Published

1981-10-16

Pages

270-4

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.6169150 View journal details

Analysis Services

Automated synthesis of gene fragments.

Contact

WeChat Official Account