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PMID: 6169638 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interspecies exchange of beta 2-microglobulin and associated MHC and differentiation antigens.

Immunogenetics ·Vol. 13 ·No. 6 ·1981-00-00 ·Pages 483-91

Schmidt W, Festenstein H, Ward PJ, Sanderson AR

Abstract

Radiolabeled human beta 2-microglobulin (beta 2m) can bind to mouse histocompatibility (H-2) antigens on the cell surface or to partially purified H-2 antigens in solution. The complexes containing human beta 2m and H-2 antigens from C3H (H-2k) mice could be immunoprecipitated specifically with alloantisera, rabbit anti-H-2 xenoantisera, and with monoclonal H-2-specific antibodies. Specific association with H-2 antigens was also observed with other haplotypes. The only exception was B10.D2 (H-2d) from which complexes containing human beta 2m could only be precipitated with anti-H-2-xenosera. Thus radiolabeled human beta 2m can be used as a specific label for mouse H-2 antigens in precipitation and radioimmunoassays. The application of this finding extends to major histocompatibility complex antigens of other species, and to differentiation antigens with primary association with beta 2m.

MeSH Terms
Animals Beta-Globulins/immunology Cross Reactions H-2 Antigens/immunology Hepatolenticular Degeneration/immunology Humans Immunosorbent Techniques Kidney Failure, Chronic/immunology Major Histocompatibility Complex Mice Mice, Inbred AKR/immunology Mice, Inbred C3H/immunology Mice, Inbred C57BL/immunology beta 2-Microglobulin/immunology
Chemicals
Beta-Globulins H-2 Antigens beta 2-Microglobulin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schmidt W
Festenstein H
Ward P J
Sanderson A R
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20 references, click to expand
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Article Info
Journal
Immunogenetics
Abbr.
Immunogenetics
ISSN
0093-7711
DOI
10.1007/BF00343716
Published
1981-00-00
Pages
483-91
Language
English
Region
United States
NLM ID
0420404
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00343716 View journal details
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