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PMID: 6170932 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract.

Nucleic acids research ·Vol. 9 ·No. 16 ·1981-08-25 ·Pages 3907-18

Dingermann T, Sharp S, Appel B, DeFranco D, Mount S, Heiermann R, Pongs O, Söll D

Abstract

We describe the preparation of a cell-free extract from Drosophila Kc cells which allows transcription of a variety of cloned eukaryotic RNA polymerase III genes. The extract has low RNA-processing nuclease activity and thus the major products obtained are primary transcripts.

MeSH Terms
Animals Cell-Free System Cloning, Molecular Drosophila/genetics In Vitro Techniques RNA/genetics RNA Polymerase III/genetics RNA, Transfer/genetics Transcription, Genetic Xenopus laevis/genetics
Chemicals
RNA RNA, Transfer RNA Polymerase III
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Dingermann T
Sharp S
Appel B
DeFranco D
Mount S
Heiermann R
Pongs O
Söll D
References (18)
18 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.16.3907
Published
1981-08-25
Pages
3907-18
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327404
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.16.3907 PMC Full Text View journal details
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