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PMID: 6170942 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Structural organization of the 16S ribosomal RNA from E. coli. Topography and secondary structure.

Nucleic acids research ·Vol. 9 ·No. 9 ·1981-05-11 ·Pages 2153-72

Stiegler P, Carbon P, Zuker M, Ebel JP, Ehresmann C

Abstract

Extensive studies in our laboratory using different ribonucleases resulted in valuable data on the topography of the E.coli 16S ribosomal RNA within the native 30S subunit, within partially unfolded 30S subunits, in the free state, and in association with individual ribosomal proteins. Such studies have precise details on the accessibility of certain residues and delineated highly accessible RNA regions. Furthermore, they provided evidence that the 16S rRNA is organized in its subunit into four distinct domains. A secondary structure model of the E.coli 16S rRNA has been derived from these topographical data. Additional information from comparative sequence analyses of the small ribosomal subunit RNAs from other species sequenced so far has been used.

MeSH Terms
Computers Escherichia coli/analysis Models, Structural Nucleic Acid Conformation Proteus vulgaris/analysis RNA, Bacterial/metabolism RNA, Ribosomal/metabolism Ribonuclease T1/metabolism Ribonucleases/metabolism Ribonucleoproteins/metabolism Zea mays/analysis
Chemicals
RNA, Bacterial RNA, Ribosomal Ribonucleoproteins Ribonucleases Ribonuclease T1
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Stiegler P
Carbon P
Zuker M
Ebel J P
Ehresmann C
References (39)
39 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.9.2153
Published
1981-05-11
Pages
2153-72
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326832
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.9.2153 PMC Full Text View journal details
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