Home LiteratureArticle Details
PMID: 6171248 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of actin polymerizaton in rat islets of Langerhans.

The Biochemical journal ·Vol. 192 ·No. 1 ·1980-10-15 ·Pages 381-3

Howell SL, Tyhurst M

Abstract

A DNAase-inhibition assay was used to determine the proportions of globular (G-) and filamentous (F-) actin in islets of Langerhans after incubation in various conditions, or after subcellular fractionation. Stimulation of insulin secretion resulted in an ATP-dependent increase in the proportion of F-actin present; fractionation showed 80-90% of the actin to be present in the final supernatant.

MeSH Terms
1-Methyl-3-isobutylxanthine/pharmacology Actins/metabolism Animals Biopolymers Dinitrophenols/pharmacology In Vitro Techniques Insulin/metabolism Insulin Secretion Islets of Langerhans/drug effects,metabolism Rats Subcellular Fractions/metabolism
Chemicals
Actins Biopolymers Dinitrophenols Insulin 1-Methyl-3-isobutylxanthine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Howell S L
Tyhurst M
References (8)
8 references, click to expand
  1. Effects of glucose concentration on incorporation of [3H]leucine into insulin using isolated mammalian islets of Langerhans.
    Biochim Biophys Acta. 1966 Dec 28;130(2):519-21 PMID: 5339718
  2. New hypothesis of insulin secretion.
    Nature. 1968 Sep 14;219(5159):1177-9 PMID: 4877537
  3. Isolation and properties of secretory granules from rat islets of Langerhans. I. Isolation of a secretory granule fraction.
    J Cell Biol. 1969 Apr;41(1):154-61 PMID: 4887227
  4. Pancreatic beta-cell web: its possible role in insulin secretion.
    Science. 1972 Mar 10;175(4026):1128-30 PMID: 4551150
  5. Association of actin and myosin with secretory granule membranes.
    Nature. 1975 Apr 10;254(5500):526-9 PMID: 1121327
  6. Muscle actin filaments bind pituitary secretory granules in vitro.
    J Cell Biol. 1977 Apr;73(1):78-87 PMID: 558196
  7. Selective assay of monomeric and filamentous actin in cell extracts, using inhibition of deoxyribonuclease I.
    Cell. 1978 Nov;15(3):935-43 PMID: 728995
  8. Interaction between insulin-storage granules and F-actin in vitro.
    Biochem J. 1979 Feb 15;178(2):367-71 PMID: 220962
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1920381
Published
1980-10-15
Pages
381-3
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1162347
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1920381 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2