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PMID: 6171773 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Secondary structure formation during RNA synthesis.

Nucleic acids research ·Vol. 9 ·No. 19 ·1981-10-10 ·Pages 5109-24

Kramer FR, Mills DR

Abstract

We observed the secondary structures that formed in an RNA molecule during its synthesis. Some of the secondary structures seen in nascent chains were observed to form, then to dissociate in favor of an alternative structure, and then to reform, as chain growth continued. The results show that secondary structures in an RNA molecule are in a state of dynamic equilibrium, and that the extension of a sequence by chain growth, or the reduction of a sequence by processing, may result in significant changes in the secondary structures that are present.

MeSH Terms
Base Sequence Electrophoresis, Polyacrylamide Gel Escherichia coli Nucleic Acid Conformation Q beta Replicase/metabolism RNA/biosynthesis Ribonuclease T1/metabolism
Chemicals
RNA Q beta Replicase Ribonuclease T1
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kramer F R
Mills D R
References (13)
13 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.19.5109
Published
1981-10-10
Pages
5109-24
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327502
Subset
IM
Grants
NCI NIH HHS · CA-23767 · United States
External Links
PubMed source DOI: 10.1093/nar/9.19.5109 PMC Full Text View journal details
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