Home LiteratureArticle Details
PMID: 6171775 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The nucleotide sequence of 5S rRNA from an extreme thermophile, Thermus thermophilus HB8.

Nucleic acids research ·Vol. 9 ·No. 19 ·1981-10-10 ·Pages 5159-62

Kumagai I, Digweed M, Erdmann VA, Watanabe K, Oshima T

Abstract

Using 3'- and 5'-end labelling sequencing techniques, the following primary structure for Thermusthermophilus HB8 5S RNA could be determined: pAA (U) CCCCCGUGCCCAUAGCGGCGUGGAACCACCCGUUCCCAUUCCGAACACGGAAGUGAAACGCGCCAGCGCC GAUGGUACUGGCGGACGACCGCUGGGAGAGUAGGUCGGUGCGGGGGA (OH). This sequence is most similar to Thermusaquaticus 5S RNA with which it shows 85% homology.

MeSH Terms
Base Sequence RNA, Bacterial RNA, Ribosomal Thermus/genetics
Chemicals
RNA, Bacterial RNA, Ribosomal
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kumagai I
Digweed M
Erdmann V A
Watanabe K
Oshima T
References (14)
14 references, click to expand
  1. The nucleotide sequence of 5S ribosomal RNA from Micrococcus lysodeikticus.
    Nucleic Acids Res. 1980 Nov 25;8(22):5423-6 PMID: 6780979
  2. Collection of published 5S and 5.8S RNA sequences and their precursors.
    Nucleic Acids Res. 1981 Jan 10;9(1):r25-42 PMID: 7010308
  3. Thermal stability of ribosomes and RNA from Thermus aquaticus.
    Biochim Biophys Acta. 1970 Apr 15;204(2):512-20 PMID: 4909656
  4. Structure and function of 5S RNA: the role of the 3' terminus in 5S RNA function.
    Mol Gen Genet. 1972;114(2):89-94 PMID: 4553099
  5. 5S RNA secondary structure.
    Nature. 1975 Aug 7;256(5517):505-7 PMID: 808733
  6. Nucleotide sequence of Thermus aquaticus ribosomal 5 S ribonucleic acid. Sequence homologies in thermophilic organisms.
    J Biol Chem. 1977 Jun 25;252(12):4256-61 PMID: 863927
  7. Rapid print-readout technique for sequencing of RNA's containing modified nucleotides.
    Nucleic Acids Res. 1979 Aug 10;6(11):3443-58 PMID: 386273
  8. A micromethod for base analysis of 32P-labeled oligoribonulcleotides.
    Anal Biochem. 1977 Nov;83(1):222-7 PMID: 920941
  9. 3'-terminal labelling of RNA with T4 RNA ligase.
    Nature. 1978 Oct 12;275(5680):560-1 PMID: 692735
  10. An enhanced thermostability in thermophilic 5-S ribonucleic acids under physiological salt conditions.
    Biochim Biophys Acta. 1978 Nov 21;521(1):288-94 PMID: 363159
  11. Evolutionary change in 5S RNA secondary structure and a phylogenic tree of 54 5S RNA species.
    Proc Natl Acad Sci U S A. 1979 Jan;76(1):381-5 PMID: 284354
  12. Use of in vitro 32P labeling in the sequence analysis of nonradioactive tRNAs.
    Methods Enzymol. 1979;59:58-109 PMID: 220499
  13. Direct chemical method for sequencing RNA.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1760-4 PMID: 377283
  14. Mapping adenines, guanines, and pyrimidines in RNA.
    Nucleic Acids Res. 1977 Aug;4(8):2527-38 PMID: 409999
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.19.5159
Published
1981-10-10
Pages
5159-62
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327506
Subset
IM
Databases
GENBANK
K01305
External Links
PubMed source DOI: 10.1093/nar/9.19.5159 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2