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PMID: 6171779 Published · ppublish English Journal Article

Antiviral activities of hybrids of two major human leukocyte interferons.

Nucleic acids research ·Vol. 9 ·No. 22 ·1981-11-25 ·Pages 6153-66

Weck PK, Apperson S, Stebbing N, Gray PW, Leung D, Shepard HM, Goeddel DV

Abstract

Four hybrid human leukocyte interferon (LeIF or IFN-alpha) genes have been constructed by in vitro recombination of LeIF-A (IFN-alpha 2) and LeIF-D (IFN-alpha 1) genes at common restriction endonuclease sites located within their coding regions. These hybrid genes have been expressed in E. coli under trp promoter control. The interferons produced [LeIF-AD (BglII), -AD (PvuII), -DA (BglII), -DA (PvuII)] have antiviral properties distinct from the parental molecules LeIF-A and -D, varying considerably in their abilities to inhibit plaque formation by different viruses in a range of mammalian cells. All six of the cloned LeIFs exhibit the heat stability, pH 2 stability and antigenic specificity of natural leukocyte interferons.

MeSH Terms
Animals Cell Line DNA Restriction Enzymes DNA, Recombinant/metabolism Encephalomyocarditis virus/drug effects Escherichia coli/genetics Genes Humans Interferons/genetics,pharmacology Leukocytes/metabolism Nucleic Acid Hybridization Operon Plasmids Simplexvirus/drug effects Vesicular stomatitis Indiana virus/drug effects
Chemicals
DNA, Recombinant Interferons DNA Restriction Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Weck P K
Apperson S
Stebbing N
Gray P W
Leung D
Shepard H M
Goeddel D V
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23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.22.6153
Published
1981-11-25
Pages
6153-66
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327591
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.22.6153 PMC Full Text View journal details
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