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PMID: 6171822 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Core (2'-5')oligoadenylate and the cordycepin analog: inhibitors of Epstein--Barr virus-induced transformation of human lymphocytes in the absence of interferon.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 11 ·1981-11-00 ·Pages 6699-703

Doetsch PW, Suhadolnik RJ, Sawada Y, Mosca JD, Flick MB, Reichenbach NL, Dang AQ, Wu JM, Charubala R, Pfleiderer W, Henderson EE

Abstract

The 3'-deoxyadenosine (cordycepin) analog of (2'-5')oligo(A) [(2'-5')oligoadenylate with a triphosphate at the 5' end], synthesized enzymatically from cordycepin 5'-triphosphate in lysed rabbit reticulocytes or L-cell extracts was (i) inhibitory to translation in lysed rabbit reticulocytes and (ii) metabolically stable in extracts of either L cells or C85-5C lymphoblasts. The 5' dephosphorylated (core) (2'-5')oligo(A) and the core cordycepin analog can replace human fibroblast interferon in preventing the transformation of human lymphocytes after infection with Epstein--Barr virus B95-8 (EBV) as determined by the decreased incorporation of [3H]thymidine into cellular DNA and the inhibition of morphological transformation of EBV-infected lymphocytes. Whereas the naturally occurring core (2'-5')oligo(A) was cytotoxic to uninfected lymphocytes and proliferating lymphoblasts, the core cordycepin analog was not. Human leukocyte interferon was more effective than human fibroblast interferon in the inhibition of EBV-induced transformation of human umbilical cord lymphocytes and adult peripheral blood lymphocytes.

MeSH Terms
Adenine Nucleotides/pharmacology Adult Cell Transformation, Viral/drug effects Cells, Cultured Deoxyadenine Nucleotides Drug Stability Female Fetal Blood Herpesvirus 4, Human/genetics Humans Infant, Newborn Interferons/pharmacology Kinetics Lymphocytes/drug effects,metabolism Oligodeoxyribonucleotides/pharmacology Oligonucleotides/pharmacology Oligoribonucleotides/pharmacology Pregnancy
Chemicals
2',5'-oligoadenylate cordycepin analog Adenine Nucleotides Deoxyadenine Nucleotides Oligodeoxyribonucleotides Oligonucleotides Oligoribonucleotides 3'-deoxyadenosine 5'-triphosphate 2',5'-oligoadenylate Interferons
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Doetsch P W
Suhadolnik R J
Sawada Y
Mosca J D
Flick M B
Reichenbach N L
Dang A Q
Wu J M
Charubala R
Pfleiderer W
Henderson E E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.11.6699
Published
1981-11-00
Pages
6699-703
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349117
Subset
IM
Grants
NCI NIH HHS · CA-12227 · United States
NCI NIH HHS · CA-23999 · United States
NIGMS NIH HHS · GM-26134 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.11.6699 PMC Full Text View journal details
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