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PMID: 6175315 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Superoxide-dependent formation of hydroxyl radicals in the presence of iron salts. Detection of 'free' iron in biological systems by using bleomycin-dependent degradation of DNA.

The Biochemical journal ·Vol. 199 ·No. 1 ·1981-10-01 ·Pages 263-5

Gutteridge JM, Rowley DA, Halliwell B

Abstract

Bleomycin in the presence of iron(II) degrades DNA to form a thiobarbituric acid-reactive product. This has been made the basis of a specific assay method for 'free' iron in biological fluids. Human synovial fluid, human cerebrospinal fluid and rat pleural-exudate fluid were found to contain micromolar concentrations of 'free' iron, which would be sufficient to allow formation of the hydroxyl radical from superoxide and hydrogen peroxide generated in vivo. This assay method does not detect iron bound to transport proteins or to enzymes.

MeSH Terms

Bleomycin/pharmacology DNA/metabolism Ferric Compounds/metabolism Humans Iron/analysis,cerebrospinal fluid Methods Pleural Effusion/analysis Superoxides/metabolism Synovial Fluid/analysis

Chemicals

Ferric Compounds Bleomycin Superoxides DNA Iron

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Gutteridge J M

Rowley D A

Halliwell B

References (14)

14 references, click to expand

Cellular mechanisms of oxygen toxicity.
Physiol Rev. 1968 Apr;48(2):311-73 PMID: 4870377
Free radicals and inflammation: protection of synovial fluid by superoxide dismutase.
Science. 1974 Aug 9;185(4150):529-31 PMID: 4841157
Superoxide-dependent production of hydroxyl radical catalyzed by iron-EDTA complex.
FEBS Lett. 1978 Feb 1;86(1):139-42 PMID: 202505
Biochemical mechanisms accounting for the toxic action of oxygen on living organisms: the key role of superoxide dismutase.
Cell Biol Int Rep. 1978 Mar;2(2):113-28 PMID: 352540
The biology of oxygen radicals.
Science. 1978 Sep 8;201(4359):875-80 PMID: 210504
Superoxide-dependent formation of hydroxyl radicals in the presence of iron chelates: is it a mechanism for hydroxyl radical production in biochemical systems?
FEBS Lett. 1978 Aug 15;92(2):321-6 PMID: 212302
Superoxide-dependent formation of hydroxyl radicals in the presence of iron salts. Its role in degradation of hyaluronic acid by a superoxide-generating system.
FEBS Lett. 1978 Dec 15;96(2):238-42 PMID: 215454
Identification of malondialdehyde as the TBA-reactant formed by bleomycin-iron free radical damage to DNA.
FEBS Lett. 1979 Sep 15;105(2):278-82 PMID: 90623
Inhibition of the iron-catalysed formation of hydroxyl radicals from superoxide and of lipid peroxidation by desferrioxamine.
Biochem J. 1979 Nov 15;184(2):469-72 PMID: 230833
Effect of oxygen-derived free radicals on hyaluronic acid.
Arthritis Rheum. 1980 Apr;23(4):455-63 PMID: 6245661
Origin of malondialdehyde from DNA degraded by Fe(II) x bleomycin.
J Biol Chem. 1980 Dec 25;255(24):11832-8 PMID: 6160153
Lactoferrin enhances hydroxyl radical production by human neutrophils, neutrophil particulate fractions, and an enzymatic generating system.
J Clin Invest. 1981 Feb;67(2):352-60 PMID: 6780607
Ethylene from 2-keto-4-thiomethyl butyric acid: the Haber-Weiss reaction.
Arch Biochem Biophys. 1980 Dec;205(2):323-9 PMID: 6258481
The role of superoxide and hydroxyl radicals in the degradation of hyaluronic acid induced by metal ions and by ascorbic acid.
J Inorg Biochem. 1981 Apr;14(2):127-34 PMID: 6265596

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1990263

Published

1981-10-01

Pages

263-5

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1163361

Subset

External Links

PubMed source DOI: 10.1042/bj1990263 PMC Full Text View journal details

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Superoxide-dependent formation of hydroxyl radicals in the presence of iron salts. Detection of 'free' iron in biological systems by using bleomycin-dependent degradation of DNA.

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