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PMID: 6176575 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

RNase III cleavage of Escherichia coli beta-galactosidase and tryptophan operon mRNA.

Journal of bacteriology ·Vol. 150 ·No. 3 ·1982-06-00 ·Pages 1489-94

Shen V, Imamoto F, Schlessinger D

Abstract

Purified RNase III of Escherichia coli cleaved the initial 479-nucleotide sequence of lac operon mRNA at four specific sites and also gave limited cleavage of trp operon mRNA. This action explains the inactivation of mRNA coding capacity by RNase III in vitro.

MeSH Terms
Base Sequence Endonucleases/metabolism Escherichia coli/metabolism Escherichia coli Proteins Lac Operon Operon RNA, Bacterial/genetics,metabolism RNA, Messenger/genetics,metabolism Ribonuclease III Ribonucleases/metabolism Tryptophan/biosynthesis beta-Galactosidase/genetics
Chemicals
Escherichia coli Proteins RNA, Bacterial RNA, Messenger Tryptophan Endonucleases Ribonucleases Ribonuclease III ribonuclease III, E coli beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shen V
Imamoto F
Schlessinger D
References (12)
12 references, click to expand
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    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3593-7 PMID: 358189
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  9. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
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    Nature. 1979 Feb 1;277(5695):407-9 PMID: 121761
  11. Functional inactivation of lac alpha-peptide mRNA by a factor that purifies that Escherichia coli RNase III.
    J Biol Chem. 1981 Feb 25;256(4):1896-902 PMID: 6257691
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
1982-06-00
Pages
1489-94
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216380
Subset
IM
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