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PMID: 6179878 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Immunological cross-reactivity in the absence of DNA homology between Pseudomonas toxin A and diphtheria toxin.

Infection and immunity ·Vol. 37 ·No. 1 ·1982-07-00 ·Pages 250-4

Sadoff JC, Buck GA, Iglewski BH, Bjorn MJ, Groman NB

Abstract

The immunodominant determinant of Pseudomonas toxin A was shown to cross-react with a normally inaccessible determinant in fragment A of diphtheria toxin. Trypsin-treated diphtheria toxin and fragment A of diphtheria toxin inhibited binding of toxin A antibody to whole toxin A, whereas whole diphtheria toxin did not inhibit this reaction. However, even at the lowest stringency no hybridization was detected between diphtheria tox probe and Pseudomonas aeruginosa DNA.

MeSH Terms
ADP Ribose Transferases Bacterial Toxins Base Sequence Cross Reactions DNA, Bacterial Diphtheria Toxin/genetics,immunology Epitopes Exotoxins/genetics,immunology Nucleic Acid Hybridization Pseudomonas aeruginosa/genetics Radioimmunoassay Virulence Factors
Chemicals
Bacterial Toxins DNA, Bacterial Diphtheria Toxin Epitopes Exotoxins Virulence Factors ADP Ribose Transferases toxA protein, Pseudomonas aeruginosa
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sadoff J C
Buck G A
Iglewski B H
Bjorn M J
Groman N B
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23 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.37.1.250-254.1982
Published
1982-07-00
Pages
250-4
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC347520
Subset
IM
Grants
NIAID NIH HHS · IAI-10492 · United States
NIAID NIH HHS · IAI-14671 · United States
External Links
PubMed source DOI: 10.1128/iai.37.1.250-254.1982 PMC Full Text View journal details
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