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PMID: 6182528 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

NMR evidence for the existence of two native conformations of 5S RNA.

Nucleic acids research ·Vol. 10 ·No. 16 ·1982-08-25 ·Pages 4973-83

Kime MJ, Moore PB

Abstract

NMR spectra of the non-exchangeable protons in 5S RNA from E. coli show the existence of two distinct conformers of the molecule which meet the operational definition of "A form" or native 5S RNA. Both are easily distinguished spectroscopically from denatured, "B form" 5S RNA. The conditions which interconvert the two A form conformers strongly suggest that the transition between them gives rise to the low temperature optical melting transition first reported in 5S RNA by Kao and Crothers (1).

MeSH Terms

Escherichia coli/analysis Magnesium Magnetic Resonance Spectroscopy Molecular Weight Nucleic Acid Conformation RNA, Bacterial RNA, Ribosomal

Chemicals

RNA, Bacterial RNA, Ribosomal Magnesium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kime M J

Moore P B

References (14)

14 references, click to expand

Role of 5S RNA in assembly and function of the 50S subunit from Escherichia coli.
Proc Natl Acad Sci U S A. 1976 Jul;73(7):2221-5 PMID: 781671
Molecular model for 5-S RNA. A small-angle x-ray scattering study of native, denatured and aggregated 5-S RNA from Escherichia coli ribosomes.
Eur J Biochem. 1976 Sep 15;68(2):481-7 PMID: 789078
A new method for the isolation of a 5 S RNA complex with proteins L5, L18 and L25 from Escherichia coli ribosomes.
FEBS Lett. 1977 Mar 1;74(2):287-91 PMID: 321249
Stoichiometry, cooperativity, and stability of interactions between 5S RNA and proteins L5, L18, and L25 from the 50S ribosomal subunit of Escherichia coli.
Biochemistry. 1978 Jun 27;17(13):2474-9 PMID: 354687
Yeast hexokinase in solution exhibits a large conformational change upon binding glucose or glucose 6-phosphate.
Biochemistry. 1979 Jan 23;18(2):338-42 PMID: 369601
Protein-RNA interactions in the bacterial ribosome.
Methods Enzymol. 1979;59:551-83 PMID: 374954
A proton-coupled conformational switch of Escherichia coli 5S ribosomal RNA.
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3360-4 PMID: 6158045
A proton NMR study of ribosomal protein L25 from Escherichia coli.
Eur J Biochem. 1981 May 15;116(2):269-76 PMID: 7018905
A hydrogen exchange study of Escherichia coli 5S RNA.
Nucleic Acids Res. 1981 Aug 25;9(16):4081-6 PMID: 6170933
Rearrangement of the conformation of Escherichia coli 5S RNA.
Proc Natl Acad Sci U S A. 1968 Sep;61(1):292-9 PMID: 4880610
Simple method for the characterization of 5S RNA.
Anal Biochem. 1972 Jan;45(1):319-25 PMID: 4621557
Size and shape of 5 S ribosomal RNA.
J Mol Biol. 1972 Oct 28;71(1):31-7 PMID: 4564301
The kinetics of renaturation of 5-S RNA from Escherichia coli in the presence of Mg 2+ ions.
Eur J Biochem. 1973 Apr;34(2):262-7 PMID: 4575977
The thermodynamics and kinetics of conformational changes in 5-S RNA from Escherichia coli.
Eur J Biochem. 1975 Sep 1;57(1):127-33 PMID: 1100399

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/10.16.4973

Published

1982-08-25

Pages

4973-83

Language

English

Region

England

NLM ID

0411011

PMCID

PMC320845

Subset

Grants

NIAID NIH HHS · AI-09167 · United States

External Links

PubMed source DOI: 10.1093/nar/10.16.4973 PMC Full Text View journal details

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NMR evidence for the existence of two native conformations of 5S RNA.

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