Home LiteratureArticle Details
PMID: 6185670 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Evidence that the contractile response of the guinea-pig ileum to capsaicin is due to release of substance P.

The Journal of physiology ·Vol. 332 ·1982-11-00 ·Pages 157-67

Barthó L, Holzer P, Lembeck F, Szolcsányi J

Abstract

1. The possible roles of substance P and opioids in the contractile response of the isolated guinea-pig ileum to the sensory stimulant drug capsaicin were investigated, and the contractions were found to be inhibited by about 60% in preparations desensitized to substance P. 2. Contractions evoked by stimulation of the mesenteric nerves in the presence of the adrenergic blocking drug guanethidine were inhibited by about 75% after the ileum had been rendered insensitive to substance P. 3. Atropine partially inhibited the effect of capsaicin. The atropine-resistant component of the contractile response to capsaicin was inhibited by more than 85% in preparations desensitized to substance P and almost abolished by the substance P antagonist, (D-Pro2,D-Trp7,9)-substance P. 4. The opioid peptide (D-Met2, Pro5)-enkephalinamide inhibited, whereas the opiate antagonist naloxone enhanced the atropine-resistant contractions in response to capsaicin. 5. The results indicate that the contractile response of the guinea-pig ileum to capsaicin and mesenteric nerve stimulation is mediated by release of substance P, presumably from sensory nerve endings in the gut. Substance P appears to act on the smooth muscle both directly and indirectly via cholinergic neurones. It is proposed that opioids modulate the non-cholinergic response to capsaicin by inhibiting the release of substance P.

MeSH Terms
Animals Atropine/pharmacology Capsaicin/pharmacology Endorphins/pharmacology Fatty Acids, Unsaturated/pharmacology Guanethidine/pharmacology Guinea Pigs Ileum/physiology In Vitro Techniques Muscle Contraction/drug effects Muscle, Smooth/physiology Naloxone/pharmacology Substance P/antagonists & inhibitors,physiology
Chemicals
Endorphins Fatty Acids, Unsaturated Substance P Naloxone Atropine Capsaicin Guanethidine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Barthó L
Holzer P
Lembeck F
Szolcsányi J
References (39)
39 references, click to expand
  1. Direct evidence for neurogenic inflammation and its prevention by denervation and by pretreatment with capsaicin.
    Br J Pharmacol Chemother. 1967 Sep;31(1):138-51 PMID: 6055248
  2. (D-met2, pro5)-enkephalinamide: a potent morphine-like analgesic.
    Eur J Pharmacol. 1977 Jun 1;43(3):293-4 PMID: 872884
  3. Opiate analgesics inhibit substance P release from rat trigeminal nucleus.
    Nature. 1977 Aug 11;268(5620):549-51 PMID: 18681
  4. The distribution of methionine-enkephalin and leucine-enkephalin in the brain and peripheral tissues.
    Br J Pharmacol. 1977 Dec;61(4):639-47 PMID: 597668
  5. Capsaicin-induced depletion of substance P from primary sensory neurones.
    Brain Res. 1978 Aug 18;152(1):183-8 PMID: 209869
  6. The site of action of capsaicin on the guinea-pig isolated ileum.
    Naunyn Schmiedebergs Arch Pharmacol. 1978 Oct;305(1):75-81 PMID: 31571
  7. New type of nerve-mediated cholinergic contractions of the guinea-pig small intestine and its selective blockade by capsaicin.
    Naunyn Schmiedebergs Arch Pharmacol. 1978 Oct;305(1):83-90 PMID: 723972
  8. Substance P and opioid interaction on stimulated and non-stimulated guinea pig ileum.
    Eur J Pharmacol. 1978 Dec 15;53(1):21-7 PMID: 738356
  9. Capsaicin-evoked release of substance P from primary sensory neurons.
    Brain Res. 1979 Jul 6;170(1):209-13 PMID: 466404
  10. Evidence for the release of endogenous substance P from intestinal nerves.
    Naunyn Schmiedebergs Arch Pharmacol. 1979 Apr;306(3):195-201 PMID: 224322
  11. Substance P release from spinal cord slices by capsaicin.
    Life Sci. 1979 Aug 13;25(7):629-36 PMID: 502756
  12. Substance P involvement in the expression of gut dependence on opiates.
    Brain Res. 1980 Jan 20;182(1):224-8 PMID: 6243235
  13. Capsaicin-sensitive innervation of the guinea-pig taenia caeci.
    Naunyn Schmiedebergs Arch Pharmacol. 1979 Oct;309(1):77-82 PMID: 522896
  14. The action of substance P on neurons of the myenteric plexus of the guinea-pig small intestine.
    Proc R Soc Lond B Biol Sci. 1979 Nov 30;206(1163):191-208 PMID: 42923
  15. Substance P as neurogenic mediator of antidromic vasodilation and neurogenic plasma extravasation.
    Naunyn Schmiedebergs Arch Pharmacol. 1979 Dec;310(2):175-83 PMID: 93706
  16. Decrease of substance P in primary afferent neurones and impairment of neurogenic plasma extravasation by capsaicin.
    Br J Pharmacol. 1980 Feb;68(2):207-13 PMID: 6153545
  17. Neurotoxic action of capsaicin on spinal substance P neurons.
    Brain Res. 1980 Mar 31;186(2):435-44 PMID: 6153557
  18. Distribution of substance P in the rat gastrointestinal tract--lack of effect of capsaicin pretreatment.
    Eur J Pharmacol. 1980 Feb 8;61(3):303-7 PMID: 6153986
  19. Distribution of enteric neurons showing immunoreactivity for substance P in the guinea-pig ileum.
    Neuroscience. 1980;5(2):323-31 PMID: 6154904
  20. Distribution of peptide- and catecholamine-containing neurons in the gastro-intestinal tract of rat and guinea-pig: immunohistochemical studies with antisera to substance P, vasoactive intestinal polypeptide, enkephalins, somatostatin, gastrin/cholecystokinin, neurotensin and dopamine beta-hydroxylase.
    Neuroscience. 1980;5(4):689-744 PMID: 6156425
  21. Intrathecal morphine inhibits substance P release from mammalian spinal cord in vivo.
    Nature. 1980 Jul 10;286(5769):155-7 PMID: 6157098
  22. Substance P-induced autodesensitization inhibits atropine-resistant, neurotensin-stimulated contractions in guinea-pig ileum.
    Eur J Pharmacol. 1980 Aug 8;65(4):461-2 PMID: 6157545
  23. Immunohistochemical studies of the enteric nervous system in tissue culture and in situ: localization of vascoactive intestinal polypeptide (VIP), substance-P and enkephalin immunoreactive nerves in the guinea-pig gut.
    Neuroscience. 1980;5(10):1717-35 PMID: 6159558
  24. Evidence that the action of cholecystokinin octapeptide on the guinea pig ileum longitudinal muscle is mediated in part by substance P release from the myenteric plexus.
    Eur J Pharmacol. 1981 Jan 5;69(1):87-93 PMID: 6162653
  25. Direct evidence for an axonal site of action of capsaicin.
    Naunyn Schmiedebergs Arch Pharmacol. 1980 Aug;313(1):91-4 PMID: 7207640
  26. Substance P immunoreactive neurons following neonatal administration of capsaicin.
    Naunyn Schmiedebergs Arch Pharmacol. 1981 Jan;315(3):185-94 PMID: 6163995
  27. Projections of substance P-containing neurons within the guinea-pig small intestine.
    Neuroscience. 1981;6(3):411-24 PMID: 6164014
  28. Immunoreactive substance P in sympathetic ganglia: distribution and sensitivity towards capsaicin.
    Neuroscience. 1981;6(3):437-41 PMID: 6164015
  29. Effects of beta-endorphin, met-enkephalin and somatostatin on the neurotensin-induced neurogenic contraction in the guinea-pig ileum.
    Regul Pept. 1981 Apr;2(1):31-42 PMID: 6112780
  30. Regional differences in the response to substance P on the longitudinal muscle and the concentration of substance P in the digestive tract of the guinea-pig.
    Neuroscience. 1981;6(7):1433-41 PMID: 6167901
  31. A specific substance P antagonist blocks smooth muscle contractions induced by non-cholinergic, non-adrenergic nerve stimulation.
    Nature. 1981 Dec 3;294(5840):467-9 PMID: 6171733
  32. Inhibition of antidromic and substance P-induced vasodilatation by a substance P antagonist.
    Acta Physiol Scand. 1981 Mar;111(3):381-2 PMID: 6171999
  33. Tetraethylammonium facilitates the stimulation-evoked loss of the enkephalins from the myenteric plexus of guinea-pig ileum.
    Proc R Soc Lond B Biol Sci. 1981 Oct 14;213(1191):171-82 PMID: 6120513
  34. Analgesic effect of antagonists of substance P.
    Biochem Biophys Res Commun. 1981 Dec 31;103(4):1318-21 PMID: 6174127
  35. Indirect evidence for the inhibition of enteric substance P neurones by opiate agonists but not by capsaicin.
    Eur J Pharmacol. 1982 Feb 5;77(4):273-9 PMID: 6174356
  36. Effects of opioids on noncholinergic excitatory responses of the guinea-pig isolated ileum: inhibition of release of enteric substance P.
    Br J Pharmacol. 1982 Jan;75(1):199-205 PMID: 6176287
  37. Substance P-like immunoreactivity in nerves associated with the vascular system of guinea-pigs.
    Neuroscience. 1982 Feb;7(2):447-59 PMID: 6176910
  38. Capsaicin applied to peripheral nerve inhibits axoplasmic transport of substance P and somatostatin.
    Brain Res. 1982 May 13;239(2):447-62 PMID: 6178469
  39. Neurally mediated contraction of ileal longitudinal muscle by substance P.
    Neurosci Lett. 1980 Apr;17(1-2):101-5 PMID: 6189007
Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1982.sp014407
Published
1982-11-00
Pages
157-67
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1197392
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.1982.sp014407 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2