Home LiteratureArticle Details
PMID: 6187935 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Antigenic variants of herpes simplex virus selected with glycoprotein-specific monoclonal antibodies.

Journal of virology ·Vol. 45 ·No. 2 ·1983-02-00 ·Pages 672-82

Holland TC, Marlin SD, Levine M, Glorioso J

Abstract

Monoclonal antibodies specific for herpes simplex virus type 1 (HSV-1) glycoproteins were used to demonstrate that HSV undergoes mutagen-induced and spontaneous antigenic variation. Hybridomas were produced by polyethylene glycol-mediated fusion of P3-X63-Ag8.653 myeloma cells with spleen cells from BALB/c mice infected with HSV-1 (strain KOS). Hybrid clones were screened for production of HSV-specific neutralizing antibody. The glycoprotein specificities of the antibodies were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of immunoprecipitates of radiolabeled infected-cell extracts. Seven hybridomas producing antibodies specific for gC, one for gB, and one for gD were characterized. All antibodies neutralized HSV-1 but not HSV-2. Two antibodies, one specific for gB and one specific for gC, were used to select viral variants resistant to neutralization by monoclonal antibody plus complement. Selections were made from untreated and bromodeoxyuridine- and nitrosoguanidine-mutagenized stocks of a plaque-purified isolate of strain KOS. After neutralization with monoclonal antibody plus complement, surviving virus was plaque purified by plating at limiting dilution and tested for resistance to neutralization with the selecting antibody. The frequency of neutralization-resistant antigenic variants selected with monoclonal antibody ranged from 4 X 10(-4) in nonmutagenized stocks to 1 X 10(-2) in mutagenized stocks. Four gC and four gB antigenic variants were isolated. Two variants resistant to neutralization by gC-specific antibodies failed to express gC, accounting for their resistant phenotype. The two other gC antigenic variants and the four gB variants expressed antigenically altered glycoproteins and were designated monoclonal-antibody-resistant, mar, mutants. The two mar C mutants were tested for resistance to neutralization with a panel of seven gC-specific monoclonal antibodies. The resulting patterns of resistance provided evidence for at least two antigenic sites on glycoprotein gC.

MeSH Terms
Antibodies, Monoclonal Antigens, Viral/genetics,immunology Epitopes Glycoproteins/genetics,immunology Isoelectric Focusing Mutation Neutralization Tests Simplexvirus/genetics,immunology
Chemicals
Antibodies, Monoclonal Antigens, Viral Epitopes Glycoproteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Holland T C
Marlin S D
Levine M
Glorioso J
References (49)
49 references, click to expand
  1. A search for viruses in smegma, premalignant and early malignant cervical tissues. The isolation of Herpesviruses with distinct antigenic properties.
    Am J Epidemiol. 1968 May;87(3):647-55 PMID: 5694774
  2. One cell-one immunoglobulin. Origin of limited heterogeneity of myeloma proteins.
    Biochem J. 1970 Jan;116(2):241-8 PMID: 5414099
  3. Proteins specified by herpes simplex virus. 3. Viruses differing in their effects on the social behavior of infected cells specify different membrane glycoproteins.
    Proc Natl Acad Sci U S A. 1970 Apr;65(4):865-71 PMID: 4314902
  4. Infection with herpes-simplex viruses 1 and 2. 1.
    N Engl J Med. 1973 Sep 27;289(13):667-74 PMID: 4353825
  5. Type-specific protein in herpes simplex virus envelope reacts with neutralising antibody.
    Nature. 1974 May 24;249(455):360-1 PMID: 4135313
  6. Proteins specified by herpes simplex virus. XII. The virion polypeptides of type 1 strains.
    J Virol. 1974 Sep;14(3):640-51 PMID: 4369085
  7. Effect of cross-immunization on monotypic antibody responses to herpes simplex virus types 1 and 2.
    J Immunol. 1976 Apr;116(4):898-903 PMID: 176275
  8. Membrane proteins specified by herpes simplex viruses. I. Identification of four glycoprotein precursors and their products in type 1-infected cells.
    J Virol. 1976 Mar;17(3):991-1008 PMID: 176453
  9. Immune interactions with cells infected with herpes simplex virus: antibodies to radioiodinated surface antigens.
    J Immunol. 1977 Jan;118(1):114-21 PMID: 187696
  10. Immunological and biochemical characterization of polypeptides induced by herpes simplex virus types 1 and 2.
    IARC Sci Publ. 1975;(11 Pt 1):63-73 PMID: 65317
  11. Cell fusion induced by herpes simplex virus is promoted and suppressed by different viral glycoproteins.
    Proc Natl Acad Sci U S A. 1977 Sep;74(9):3913-7 PMID: 198812
  12. Identification of a common antigen of herpes simplex virus bovine herpes mammillitis virus, and B virus.
    J Virol. 1978 Jun;26(3):712-7 PMID: 78988
  13. Mouse myeloma--spleen cell hybrids: Enhanced hybridization frequencies and rapid screening procedures.
    Curr Top Microbiol Immunol. 1978;81:107-9 PMID: 567553
  14. Type-common CP-1 antigen of herpes simplex virus is associated with a 59,000-molecular-weight envelope glycoprotein.
    J Virol. 1978 Jul;27(1):172-81 PMID: 80458
  15. Clonal nature of the immune response to phosphocholine. VI. Molecular uniformity of a single idiotype among BALB/c mice.
    J Immunol. 1978 Oct;121(4):1410-5 PMID: 701802
  16. Antigenic drift in influenza A viruses. I. Selection and characterization of antigenic variants of A/PR/8/34 (HON1) influenza virus with monoclonal antibodies.
    J Exp Med. 1978 Aug 1;148(2):383-92 PMID: 359746
  17. The natural history of recurrent facial-oral infection with herpes simplex virus.
    J Infect Dis. 1978 Dec;138(6):897-905 PMID: 216753
  18. Immunoelectrophoretic identification and purification of herpes simplex virus antigens released from infected cells in tissue culture.
    Intervirology. 1979;11(2):104-10 PMID: 85610
  19. Antigenic variants of rabies virus.
    J Exp Med. 1980 Jul 1;152(1):99-112 PMID: 6156982
  20. Mutant analysis of herpes simplex virus-induced cell surface antigens: resistance to complement-mediated immune cytolysis.
    J Virol. 1980 Sep;35(3):672-81 PMID: 6252331
  21. Cell-mediated immunity to herpes simplex virus: specificity of cytotoxic T cells.
    Infect Immun. 1980 Nov;30(2):451-61 PMID: 6969228
  22. Serological analysis of antigenic determinants on the env gene products of AKR dualtropic (MCF) murine leukemia viruses.
    Virology. 1980 Nov;107(1):81-8 PMID: 6160678
  23. Structural identification of the antibody-binding sites of Hong Kong influenza haemagglutinin and their involvement in antigenic variation.
    Nature. 1981 Jan 29;289(5796):373-8 PMID: 6162101
  24. Type-common and type-specific monoclonal antibody to herpes simplex virus type 1.
    Infect Immun. 1980 Aug;29(2):724-32 PMID: 6260657
  25. Herpes simplex virus antigens and antibodies: a survey of studies based on quantitative immunoelectrophoresis.
    Rev Infect Dis. 1980 Nov-Dec;2(6):899-913 PMID: 6164087
  26. Method for induction of mutations in physically defined regions of the herpes simplex virus genome.
    J Virol. 1981 Apr;38(1):41-9 PMID: 6264113
  27. Mechanism of antigenic drift in influenza virus. Amino acid sequence changes in an antigenically active region of Hong Kong (H3N2) influenza virus hemagglutinin.
    J Mol Biol. 1981 Jan 15;145(2):339-61 PMID: 6167724
  28. Topological mapping antigenic sites on the influenza A/PR/8/34 virus hemagglutinin using monoclonal antibodies.
    Virology. 1981 Aug;113(1):64-72 PMID: 6791373
  29. Antigenic characterization of viruses by monoclonal antibodies.
    Annu Rev Microbiol. 1981;35:185-206 PMID: 6170247
  30. Serological analysis of herpes simplex virus types 1 and 2 with monoclonal antibodies.
    Infect Immun. 1982 Jan;35(1):363-7 PMID: 6172383
  31. Monoclonal antibodies to herpes simplex virus type 1 proteins, including the immediate-early protein ICP 4.
    Infect Immun. 1981 Dec;34(3):684-92 PMID: 6277788
  32. Molecular mechanisms of variation in influenza viruses.
    Nature. 1982 Mar 11;296(5853):115-21 PMID: 6174870
  33. Variation of influenza A, B, and C viruses.
    Science. 1982 Mar 19;215(4539):1468-74 PMID: 7038875
  34. Evidence for functional domains on the reovirus type 3 hemagglutinin.
    Virology. 1982 Feb;117(1):146-55 PMID: 6175082
  35. Effect of monoclonal antibodies on limited proteolysis of native glycoprotein gD of herpes simplex virus type 1.
    J Virol. 1982 Feb;41(2):478-88 PMID: 6176725
  36. Immunological speculations.
    Annu Rev Microbiol. 1960;14:341-58 PMID: 13789973
  37. Molecular genetics of herpes simplex virus. II. Mapping of the major viral glycoproteins and of the genetic loci specifying the social behavior of infected cells.
    J Virol. 1979 Feb;29(2):677-97 PMID: 219254
  38. Antigenic drift in type A influenza virus: peptide mapping and antigenic analysis of A/PR/8/34 (HON1) variants selected with monoclonal antibodies.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1425-9 PMID: 86990
  39. The immunology of primary and recurrent herpesvirus infection: an overview.
    IARC Sci Publ. 1978;(24 Pt 2):659-73 PMID: 221390
  40. Fine specificity of regulatory T cells. II. Suppressor and helper T cells are induced by different regions of hen egg-white lysozyme in a genetically nonresponder mouse strain.
    J Exp Med. 1979 Aug 1;150(2):293-306 PMID: 88500
  41. A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.
    J Immunol. 1979 Oct;123(4):1548-50 PMID: 113458
  42. Herpes simplex virus glycoproteins: participation of individual herpes simplex virus type 1 glycoprotein antigens in immunocytolysis and their correlation with previously identified glycopolypeptides.
    J Virol. 1979 Dec;32(3):741-8 PMID: 229263
  43. Membrane proteins specified by herpes simplex viruses. V. Identification of an Fc-binding glycoprotein.
    J Virol. 1979 Dec;32(3):779-89 PMID: 229267
  44. Antigenic variants of influenza viruses: marked differences in the frequencies of variants selected with different monoclonal antibodies.
    Virology. 1980 Apr 30;102(2):458-62 PMID: 6154380
  45. Use of hybridoma monoclonal antibodies in the detection of antigenic differences between rabies and rabies-related virus proteins. II. The glycoprotein.
    J Gen Virol. 1980 May;48(1):105-9 PMID: 6155432
  46. On topographic antigenic determinants in myoglobins.
    Mol Immunol. 1980 Apr;17(4):519-25 PMID: 6156406
  47. The role of viral glycoproteins in adsorption, penetration, and pathogenicity of viruses.
    Rev Infect Dis. 1980 Jan-Feb;2(1):40-61 PMID: 6994202
  48. Viral glycoproteins in infectious disease processes.
    Rev Infect Dis. 1980 Jan-Feb;2(1):78-103 PMID: 6994204
  49. Determination of the number of nonoverlapping antigenic areas on Hong Kong (H3N2) influenza virus hemagglutinin with monoclonal antibodies and the selection of variants with potential epidemiological significance.
    Virology. 1980 Jul 15;104(1):139-48 PMID: 6156537
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.45.2.672-682.1983
Published
1983-02-00
Pages
672-82
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256462
Subset
IM
Grants
NIAID NIH HHS · AI17900 · United States
NIAID NIH HHS · AI18228 · United States
NCRR NIH HHS · RR00200 · United States
External Links
PubMed source DOI: 10.1128/JVI.45.2.672-682.1983 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2