Improved section adhesion for immunocytochemistry using high molecular weight polymers of L-lysine as a slide coating.

Histochemistry ·Vol. 77 ·No. 2 ·1983-00-00 ·Pages 275-9

Huang WM, Gibson SJ, Facer P, Gu J, Polak JM

Abstract

Poly-L-lysine (PPL) has been used to coat glass slides in the preparation of tissue sections for immunocytochemical staining. The adhesive properties of different molecular weight (m.w.) polymers of L-lysine have been tested on pre-fixed cryostat sections which were subjected to a 3 day washing treatment. It has been found that the higher the molecular weight of the polymer, the greater the adhesive force it provides. PLL (m.w. 350,000) at concentrations in the range of 0.05-0.1% was found to be the most effective polymer.

MeSH Terms

Adhesiveness Animals Frozen Sections Immunochemistry/methods Molecular Weight Peptides Polylysine Staining and Labeling

Chemicals

Peptides Polylysine

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Huang W M

Gibson S J

Facer P

Gu J

Polak J M

References (6)

6 references, click to expand

Repeated application of first-layer antiserum improves immunofluorescence staining: a modification of the indirect immunofluorescence staining procedure.
Histochem J. 1983 May;15(5):475-82 PMID: 6192112
Use of polylysine-coated slides in preparation of cell samples for diagnostic cytology with special reference to urine sample.
J Clin Pathol. 1980 Mar;33(3):309-11 PMID: 7381026
Experimental immunohistochemical studies on the localization and distribution of substance P in cat primary sensory neurons.
Brain Res. 1975 Dec 19;100(2):235-52 PMID: 1104079
The distribution of nine peptides in rat spinal cord with special emphasis on the substantia gelatinosa and on the area around the central canal (lamina X).
J Comp Neurol. 1981 Sep 1;201(1):65-79 PMID: 6168670
Adhesion of cells to surfaces coated with polylysine. Applications to electron microscopy.
J Cell Biol. 1975 Jul;66(1):198-200 PMID: 1095595
Insolubilization of protein antigens on polyacrylic plastic beads using poly-L-lysine.
J Immunol Methods. 1976;12(1-2):81-9 PMID: 792353

Article Info

Journal

Histochemistry

Abbr.

Histochemistry

ISSN

0301-5564

DOI

10.1007/BF00506570

Published

1983-00-00

Pages

275-9

Language

English

Region

Germany

NLM ID

0411300

Subset

External Links

PubMed source DOI: 10.1007/BF00506570

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Improved section adhesion for immunocytochemistry using high molecular weight polymers of L-lysine as a slide coating.

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