Passage of serum-destined proteins through the Golgi apparatus of rat liver. An examination of heavy and light Golgi fractions.

The Journal of cell biology ·Vol. 76 ·No. 1 ·1978-01-00 ·Pages 87-97

Bergeron JJ, Borts D, Cruz J

Abstract

The participation of hepatic Golgi apparatus in the intracellular transport of blood-destined proteins has been analyzed using Golgi fractions enriched in cis and trans components of the Golgi apparatus. SDS-polyacrylamide gel electrophoresis of the liver Golgi fractions showed several proteins corresponding in relative proportions and mobilities with serum proteins. After a pulse injection of labeled leucine, the secretory content of the cis Golgi fraction was labeled earlier than the trans Golgi fraction. Taken together, the results show the participation of the liver Golgi apparatus in the secretion of most of the serum proteins and provide documentation for a sequential progression of secretory protein through the cis and trans components of the Golgi apparatus.

MeSH Terms

Animals Biological Transport Blood Proteins/metabolism Golgi Apparatus/metabolism,ultrastructure Kinetics Liver/metabolism,ultrastructure Male Microscopy, Electron Molecular Weight Rats

Chemicals

Blood Proteins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Bergeron J J

Borts D

Cruz J

References (24)

24 references, click to expand

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Article Info

Journal

The Journal of cell biology

Abbr.

J Cell Biol

ISSN

0021-9525

DOI

10.1083/jcb.76.1.87

Published

1978-01-00

Pages

87-97

Language

English

Region

United States

NLM ID

0375356

PMCID

PMC2109960

Subset

External Links

PubMed source DOI: 10.1083/jcb.76.1.87 PMC Full Text View journal details

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Passage of serum-destined proteins through the Golgi apparatus of rat liver. An examination of heavy and light Golgi fractions.

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