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PMID: 6189127 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Coexpression of keratin- and vimentin-type intermediate filaments in human metastatic carcinoma cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 9 ·1983-05-00 ·Pages 2618-22

Ramaekers FC, Haag D, Kant A, Moesker O, Jap PH, Vooijs GP

Abstract

Metastatic tumor cells of epithelial origin present in effusions from human serous cavity fluids (ascites or pleural fluid) were examined for their intermediate-sized filament types by using antibodies to keratin, vimentin, and desmin in the indirect immunofluorescence technique. Solid epithelial tumors (both primary carcinomas and their metastases) contain keratin intermediate-sized filaments exclusively. However, when these cells are present in ascitic or pleural fluid, they also express vimentin, which occurs in a fibrillar organization. The possible effects of this additional, but temporary, cytoskeleton on metastatic growth or aggressiveness (or both) are discussed.

MeSH Terms
Carcinoma/ultrastructure Cytoskeleton/analysis,ultrastructure Fluorescent Antibody Technique Humans Intermediate Filament Proteins/analysis Keratins/analysis Neoplasm Metastasis Neoplasms/ultrastructure Vimentin
Chemicals
Intermediate Filament Proteins Vimentin Keratins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ramaekers F C
Haag D
Kant A
Moesker O
Jap P H
Vooijs G P
References (28)
28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.9.2618
Published
1983-05-00
Pages
2618-22
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393878
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.80.9.2618 PMC Full Text View journal details
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