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PMID: 6191330 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Antigenically distinct subpopulations of myeloid progenitor cells (CFU-GM) in human peripheral blood and marrow.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 13 ·1983-07-00 ·Pages 4114-8

Ferrero D, Broxmeyer HE, Pagliardi GL, Venuta S, Lange B, Pessano S, Rovera G

Abstract

Two types of progenitor cells of the human granulocytic and monocytic lineages (CFU-GM) can be distinguished by using mouse monoclonal antibodies against human hemopoietic cells. Type 1 CFU-GM contribute all of the peripheral blood CFU-GM as well as a small fraction of bone marrow CFU-GM and express surface antigens recognized by "anti-lymphomonocytic" monoclonal antibodies S3-13 and S17-25 but not the antigens recognized by R1B19 and WGHS-29-1 (two monoclonal antibodies that react with all the cells of the granulocytic lineage). Type 2 CFU-GM are present only in the marrow and react with S3-13, R1B19, and WGHS-29-1. Partial reactivity with S17-25 was observed only in the complement-dependent cytotoxicity test. In vitro culture of type 1 CFU-GM in liquid medium in the presence of granulocyte-macrophage colony-stimulatory factor (GM-CSF) generates colony-forming cells that have the surface phenotype of type 2 CFU-GM. This finding supports the idea of two different stages of maturation of myelomonocytic progenitor cells represented by type 1 and type 2 CFU-GM.

MeSH Terms
Adult Antibodies, Monoclonal Antigens, Surface/analysis Bone Marrow/immunology Cell Separation Complement System Proteins/immunology Cytotoxicity, Immunologic Epitopes/analysis Flow Cytometry Hematopoietic Stem Cells/immunology Humans Leukocytes/immunology Phenotype T-Lymphocytes/immunology
Chemicals
Antibodies, Monoclonal Antigens, Surface Epitopes Complement System Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ferrero D
Broxmeyer H E
Pagliardi G L
Venuta S
Lange B
Pessano S
Rovera G
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.13.4114
Published
1983-07-00
Pages
4114-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC394211
Subset
IM
Grants
NCI NIH HHS · CA10815 · United States
NCI NIH HHS · CA21124 · United States
NCI NIH HHS · CA23528 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.13.4114 PMC Full Text View journal details
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