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PMID: 6191757 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Effect of starvation and refeeding on polyamine concentrations and ornithine decarboxylase antizyme in mammary gland of lactating rats.

The Biochemical journal ·Vol. 212 ·No. 1 ·1983-04-15 ·Pages 149-53

Brosnan ME, Farrell R, Wilansky H, Williamson DH

Abstract

Starvation caused a marked increase in putrescine content in mammary gland of lactating rats, together with a marked decrease in activity of ornithine decarboxylase and appearance of measurable ornithine decarboxylase antizyme. 2. Refeeding for 5 h caused disappearance of free antizyme and ornithine decarboxylase activity returned to the value in fed animals. Putrescine concentration remained elevated. 3. There was no significant change in nucleic acid content of mammary gland from starved rats, but spermidine and spermine contents increased significantly. 4. Refeeding for 5 h returned the spermidine content of mammary glands to 'fed' values, and significantly decreased the content of spermine, although it did not reach control values. Thus changes in polyamine content of mammary gland in starved rats are clearly dissociated from changes in either RNA content or activities of polyamine-synthetic decarboxylases. 5. Starvation caused a fall in the content of spermidine in liver, with no change in spermine content. Refeeding for 5 h returned the spermidine content to 'fed' values.

MeSH Terms
Animals Carboxy-Lyases/antagonists & inhibitors DNA/metabolism Female Food Lactation Liver/metabolism Mammary Glands, Animal/enzymology,metabolism Ornithine Decarboxylase Inhibitors Polyamines/metabolism Pregnancy Proteins/metabolism RNA/metabolism Rats Rats, Inbred Strains Starvation/metabolism
Chemicals
Ornithine Decarboxylase Inhibitors Polyamines Proteins ornithine decarboxylase antizyme RNA DNA Carboxy-Lyases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Brosnan M E
Farrell R
Wilansky H
Williamson D H
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21 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2120149
Published
1983-04-15
Pages
149-53
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1152022
Subset
IM
Grants
NIADDK NIH HHS · AM 11748 · United States
External Links
PubMed source DOI: 10.1042/bj2120149 PMC Full Text View journal details
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