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PMID: 6192323 Published · ppublish English Journal Article

Neural tissues express high levels of the cellular src gene product pp60c-src.

Molecular and cellular biology ·Vol. 3 ·No. 6 ·1983-06-00 ·Pages 1157-62

Cotton PC, Brugge JS

Abstract

Chicken embryo tissues were examined for the expression of pp60c-src, the normal cellular homolog of the transforming protein of Rous sarcoma virus. Three assays, including a solid-phase radioimmunoassay, a competitive radioimmunoprecipitation assay, and an immune complex protein kinase assay, were employed. Elevated levels of pp60c-src were detected in lysates from several neural tissues, including brain, retina, and spinal ganglia. Other tissues contained 8- to 10-fold-lower levels of pp60c-src, levels comparable to those found in chicken embryo fibroblasts. Expression of pp60c-src in brain tissues was also shown to vary with the developmental stage of the embryo.

MeSH Terms
Age Factors Animals Brain/embryology,metabolism Chick Embryo Nerve Tissue Proteins/metabolism Oncogene Protein pp60(v-src) Phosphotyrosine Protein Kinases/metabolism Tyrosine/analogs & derivatives,biosynthesis Viral Proteins/metabolism
Chemicals
Nerve Tissue Proteins Viral Proteins Phosphotyrosine Tyrosine Protein Kinases Oncogene Protein pp60(v-src)
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cotton P C
Brugge J S
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28 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.3.6.1157-1162.1983
Published
1983-06-00
Pages
1157-62
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368645
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.3.6.1157-1162.1983 PMC Full Text View journal details
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