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PMID: 6193735 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Detection of cellulase activity in polyacrylamide gels using Congo red-stained agar replicas.

Analytical biochemistry ·Vol. 131 ·No. 2 ·1983-06-00 ·Pages 333-6

Béguin P

Abstract

Bands that have cellulolytic activity are visualized after polyacrylamide gel electrophoresis by laying the slab gel on top of a thin sheet of 2% agar containing 0.1% carboxymethylcellulose. After a suitable incubation time, zones of carboxymethylcellulose hydrolysis are revealed by staining the agar replica with Congo red.

MeSH Terms
Agar Carboxymethylcellulose Sodium Cellulase/analysis,isolation & purification Congo Red Electrophoresis, Polyacrylamide Gel/methods Staining and Labeling
Chemicals
Congo Red Agar Cellulase Carboxymethylcellulose Sodium
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Béguin P
Article Info
Journal
Analytical biochemistry
Abbr.
Anal Biochem
ISSN
0003-2697
DOI
10.1016/0003-2697(83)90178-1
Published
1983-06-00
Pages
333-6
Language
English
Region
United States
NLM ID
0370535
Subset
IM
External Links
PubMed source DOI: 10.1016/0003-2697(83)90178-1 View journal details
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