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PMID: 6196191 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Coronavirus mRNA synthesis involves fusion of non-contiguous sequences.

The EMBO journal ·Vol. 2 ·No. 10 ·1983-00-00 ·Pages 1839-44

Spaan W, Delius H, Skinner M, Armstrong J, Rottier P, Smeekens S, van der Zeijst BA, Siddell SG

Abstract

Positive-stranded genomic RNA of coronavirus MHV and its six subgenomic mRNAs are synthesized in the cytoplasm of the host cell. The mRNAs are composed of leader and body sequences which are non-contiguous on the genome and are fused together in the cytoplasm by a mechanism which appears to involve an unusual and specific 'polymerase jumping' event.

MeSH Terms
Base Sequence Coronaviridae/genetics DNA/analysis,metabolism Genes, Viral Microscopy, Electron Nucleic Acid Hybridization RNA, Messenger/genetics RNA, Viral/genetics RNA-Directed DNA Polymerase
Chemicals
RNA, Messenger RNA, Viral DNA RNA-Directed DNA Polymerase
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Spaan W
Delius H
Skinner M
Armstrong J
Rottier P
Smeekens S
van der Zeijst B A
Siddell S G
References (34)
34 references, click to expand
  1. Length measurements of RNA synthesized in vitro by Escherichia coli RNA polymerase.
    J Mol Biol. 1973 Mar 15;74(4):677-87 PMID: 4354077
  2. Coronavirus JHM: nucleotide sequence of the mRNA that encodes nucleocapsid protein.
    Nucleic Acids Res. 1983 Aug 11;11(15):5045-54 PMID: 6308569
  3. Sequence of chicken ovalbumin mRNA.
    Nature. 1978 Jun 29;273(5665):723-8 PMID: 661981
  4. 3'-terminal nucleotide sequence of encephalomyocarditis virus RNA determined by reverse transcriptase and chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1978 Sep;75(9):4257-61 PMID: 81487
  5. Sequences from the beginning of the fiber messenger RNA of adenovirus-2.
    J Mol Biol. 1979 Jun 25;131(2):341-52 PMID: 318165
  6. Coronavirus JHM: cell-free synthesis of structural protein p60.
    J Virol. 1980 Jan;33(1):10-7 PMID: 7365865
  7. DNA sequence analysis by primed synthesis.
    Methods Enzymol. 1980;65(1):560-80 PMID: 6154869
  8. Electron microscopic mapping and sequence analysis of the terminator for the early message of E. coli phage T7.
    Mol Gen Genet. 1980 Apr;178(1):27-34 PMID: 6247619
  9. Isolation and identification of virus-specific mRNAs in cells infected with mouse hepatitis virus (MHV-A59).
    Virology. 1981 Jan 30;108(2):424-34 PMID: 6258295
  10. The replication of murine coronaviruses in enucleated cells.
    Virology. 1981 Apr 15;110(1):225-30 PMID: 6259831
  11. Translation of three mouse hepatitis virus strain A59 subgenomic RNAs in Xenopus laevis oocytes.
    J Virol. 1981 Apr;38(1):20-6 PMID: 6264100
  12. Comparative analysis of RNA genomes of mouse hepatitis viruses.
    J Virol. 1981 May;38(2):661-70 PMID: 6165837
  13. Intracellular murine hepatitis virus-specific RNAs contain common sequences.
    Virology. 1981 Jul 30;112(2):596-604 PMID: 6114592
  14. Synthesis of subgenomic mRNA's of mouse hepatitis virus is initiated independently: evidence from UV transcription mapping.
    J Virol. 1981 Aug;39(2):401-6 PMID: 6268831
  15. The virus-specific intracellular RNA species of two murine coronaviruses: MHV-a59 and MHV-JHM.
    Virology. 1981 Oct 15;114(1):39-51 PMID: 7281517
  16. Origin and replication of defective interfering particles.
    Curr Top Microbiol Immunol. 1981;93:151-207 PMID: 7026180
  17. Splicing and the regulation of viral gene expression.
    Curr Top Microbiol Immunol. 1981;93:47-79 PMID: 6269804
  18. Mechanism of mRNA recognition by eukaryotic ribosomes during initiation of protein synthesis.
    Curr Top Microbiol Immunol. 1981;93:81-123 PMID: 7026182
  19. Mouse hepatitis virus A59: mRNA structure and genetic localization of the sequence divergence from hepatotropic strain MHV-3.
    J Virol. 1981 Sep;39(3):823-34 PMID: 6169842
  20. Improvements of DNA sequencing gels.
    Anal Biochem. 1981 Aug;115(2):450-7 PMID: 7304971
  21. Host cell nuclear function and murine hepatitis virus replication.
    J Gen Virol. 1981 Oct;56(Pt 2):457-60 PMID: 6171615
  22. The origins of defective interfering particles of the negative-strand RNA viruses.
    Cell. 1981 Oct;26(2 Pt 2):145-54 PMID: 7037195
  23. Nucleotide sequences of influenza virus segments 1 and 3 reveal mosaic structure of a small viral RNA segment.
    Cell. 1982 Feb;28(2):303-13 PMID: 7060132
  24. Further characterization of mRNA's of mouse hepatitis virus: presence of common 5'-end nucleotides.
    J Virol. 1982 Feb;41(2):557-65 PMID: 6281467
  25. Sequence relationships between the genome and the intracellular RNA species 1, 3, 6, and 7 of mouse hepatitis virus strain A59.
    J Virol. 1982 May;42(2):432-9 PMID: 6283166
  26. Cell-free translation of murine coronavirus RNA.
    J Virol. 1982 Sep;43(3):905-13 PMID: 6292469
  27. Replication of mouse hepatitis virus: negative-stranded RNA and replicative form RNA are of genome length.
    J Virol. 1982 Nov;44(2):487-92 PMID: 6292513
  28. Recombination in RNA.
    Cell. 1982 Jul;29(3):921-8 PMID: 6295637
  29. RNA-dependent RNA polymerase activity in murine coronavirus-infected cells.
    J Gen Virol. 1983 Jan;64 (Pt 1):103-11 PMID: 6296295
  30. Coronavirus JHM: coding assignments of subgenomic mRNAs.
    J Gen Virol. 1983 Jan;64 (Pt 1):113-25 PMID: 6130122
  31. The biology of coronaviruses.
    J Gen Virol. 1983 Apr;64 (Pt 4):761-76 PMID: 6300299
  32. Sequence of the nucleocapsid gene from murine coronavirus MHV-A59.
    Nucleic Acids Res. 1983 Feb 11;11(3):883-91 PMID: 6687635
  33. Presence of leader sequences in the mRNA of mouse hepatitis virus.
    J Virol. 1983 Jun;46(3):1027-33 PMID: 6304334
  34. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
1839-44
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555368
Subset
IM
Databases
GENBANK
J02252, K00757, X00509
External Links
PubMed source PMC Full Text View journal details
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