Crystallization of a ribonuclease-resistant fragment of Escherichia coli 5 S ribosomal RNA and its complex with protein L25.

A ribonuclease-resistant fragment of Escherichia coli 5 S ribosomal RNA has been crystallized. The space group is P6(1)22 or P6(5)22, with a = 59.5 A and C = 268 A. The crystals contain one molecule per asymmetric unit, and show diffraction to 4.0 A resolution. Also, a complex of this fragment with L25 ribosomal protein has been crystallized in the same space group, but with a = 119 A, c = 250 A and four molecules per asymmetric unit.