Abstract
In order to determine the molecular basis of adenosine deaminase (ADA) deficiency in cells derived from patients with severe combined immunodeficiency (SCID) disease, we used a human ADA cDNA clone (1) to analyse the organization and transcription of the ADA gene in both normal and ADA-SCID cells. In five lymphoblastoid ADA-SCID cell lines we could detect no deletions or rearrangements in the ADA gene and its flanking sequences. Furthermore, synthesis and processing of ADA mRNA appeared to be normal in the ADA-SCID cells, and ADA-specific mRNA from two ADA-SCID cells could be translated in vitro into a protein with the molecular weight of normal ADA; this protein, however, could hardly be precipitated with an ADA antiserum. The results indicate that in these two ADA-SCID cell lines, the lack of ADA activity is not due to transcriptional or translational defects, but to subtle changes in the configuration of the protein affecting both its enzymatic and immunological characteristics.
MeSH Terms
Adenosine Deaminase/deficiency,genetics
Cell Line
Chromosome Deletion
Cloning, Molecular
DNA/analysis
Genes
Humans
Immunologic Deficiency Syndromes/enzymology,genetics
Nucleic Acid Hybridization
Nucleoside Deaminases/deficiency
Poly A/genetics
RNA/genetics
RNA, Messenger/genetics
Transcription, Genetic
Chemicals
RNA, Messenger
Poly A
RNA
DNA
Nucleoside Deaminases
Adenosine Deaminase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Valerio D
Duyvesteyn M G
van Ormondt H
Meera Khan P
van der Eb A J
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