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PMID: 6199344 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Unusual rRNA-linked complex of 50S ribosomal subunits isolated from an Escherichia coli RNase III mutant.

Journal of bacteriology ·Vol. 157 ·No. 3 ·1984-03-00 ·Pages 971-4

Clark MW, Lake JA

Abstract

We have isolated and characterized complexes of ribosomal subunits from Escherichia coli mutant AB301-105 connected by strands of unprocessed RNA. By electron microscopy of these complexes, the location of the 5' end of 5S RNA was established and the location of the 3' end of 23S RNA was confirmed. We also note that in these complexes insertion of 5S rRNA can proceed without the 23S-5S spacer having been processed.

MeSH Terms
Endoribonucleases/metabolism Escherichia coli/genetics,metabolism,ultrastructure Escherichia coli Proteins Microscopy, Electron Mutation RNA Processing, Post-Transcriptional RNA, Bacterial/analysis,metabolism RNA, Ribosomal/analysis,metabolism Ribonuclease III Ribosomes/analysis,ultrastructure Transcription, Genetic
Chemicals
Escherichia coli Proteins RNA, Bacterial RNA, Ribosomal Endoribonucleases Ribonuclease III ribonuclease III, E coli
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Clark M W
Lake J A
References (19)
19 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.157.3.971-974.1984
Published
1984-03-00
Pages
971-4
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215361
Subset
IM
Grants
PHS HHS · 07104 · United States
NIGMS NIH HHS · GM24034 · United States
External Links
PubMed source DOI: 10.1128/jb.157.3.971-974.1984 PMC Full Text View journal details
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