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PMID: 6201324 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Silver staining the chromosome scaffold.

Chromosoma ·Vol. 89 ·No. 3 ·1984-00-00 ·Pages 186-92

Earnshaw WC, Laemmli UK

Abstract

Cytological silver-staining procedures reveal the presence of a "core" running along the chromatid axes of isolated HeLa mitotic chromosomes. In this communication we examine the relationship between this "core" and the nonhistone chromosome scaffolding, isolated and characterized in previous publications from this laboratory. When chromosomes on coverslips were subjected to the steps used for scaffold isolation in vitro and subsequently stained with silver, the characteristic "core" staining was unaffected. Control experiments suggested that the "core" does not contain large amounts of DNA. When scaffolds were isolated in vitro, centrifuged onto electron microscope grids, and stained with silver, they were found to stain selectively under conditions where specific "core" staining was observed in intact chromosomes. These results suggest that the nonhistone scaffolding is the principal target of the silver stain in chromosomes.

MeSH Terms
Chromosomes/ultrastructure HeLa Cells Humans Microscopy, Fluorescence Mitosis Silver Staining and Labeling
Chemicals
Silver
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Earnshaw W C
Laemmli U K
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47 references, click to expand
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00294997
Published
1984-00-00
Pages
186-92
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00294997 View journal details
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