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PMID: 6201749 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interleukin-3 supports growth of mouse pre-B-cell clones in vitro.

Nature ·Vol. 309 ·No. 5964 ·1984-00-00 ·Pages 126-31

Palacios R, Henson G, Steinmetz M, McKearn JP

Abstract

Study of the differentiation of immunoglobulin-producing B lymphocytes has been hampered by the inability to maintain homogeneous populations of precursor cells in vitro. We describe here that interleukin-3 supports the growth of freshly isolated fetal liver pre-B cells and the long-term culture of interleukin-3 dependent pre-B-cell clones that can be induced to mature into antibody secreting cells in vitro.

MeSH Terms

Animals Antigens, Surface/analysis B-Lymphocytes/drug effects,immunology Cell Differentiation Cell Line Clone Cells DNA Replication/drug effects Epitopes/analysis Genes Immunoglobulin M/analysis Immunoglobulin kappa-Chains/genetics Immunoglobulin mu-Chains/genetics Interleukin-3 Lymphocyte Activation Lymphokines/pharmacology Mice Mice, Nude Phenotype

Chemicals

Antigens, Surface Epitopes Immunoglobulin M Immunoglobulin kappa-Chains Immunoglobulin mu-Chains Interleukin-3 Lymphokines

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Palacios R

Henson G

Steinmetz M

McKearn J P

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/309126a0

Published

1984-00-00

Pages

126-31

Language

English

Region

England

NLM ID

0410462

Subset

External Links

PubMed source DOI: 10.1038/309126a0 View journal details

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Interleukin-3 supports growth of mouse pre-B-cell clones in vitro.

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