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PMID: 6201848 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A unique mechanism regulating gene expression: translational inhibition by a complementary RNA transcript (micRNA).

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 7 ·1984-04-00 ·Pages 1966-70

Mizuno T, Chou MY, Inouye M

Abstract

The expression of the genes for the major outer membrane proteins OmpF and OmpC are osmoregulated. The ompC locus was found to be transcribed bidirectionally under conditions of high osmolarity and a 174-base transcript encoded upstream of ompC was found to inhibit the OmpF production and to substantially reduce the amount of the ompF mRNA. This RNA [mRNA-interfering complementary RNA (micRNA)] has a long sequence that is complementary to the 5' end region of the ompF mRNA. We propose that the micRNA inhibits the translation of the ompF mRNA by hybridizing with it. This RNA interaction may cause premature termination of the transcription of the ompF gene or destabilization of the ompF mRNA or both.

MeSH Terms
Bacterial Outer Membrane Proteins Base Sequence DNA Restriction Enzymes Escherichia coli/genetics Genes Genes, Bacterial Membrane Proteins/genetics Nucleic Acid Conformation Nucleic Acid Hybridization Plasmids Protein Biosynthesis/drug effects RNA/pharmacology Transcription, Genetic
Chemicals
Bacterial Outer Membrane Proteins Membrane Proteins RNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mizuno T
Chou M Y
Inouye M
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.7.1966
Published
1984-04-00
Pages
1966-70
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345417
Subset
IM
Grants
NIGMS NIH HHS · GM19043 · United States
Databases
GENBANK
K00541, M10314, M14188
External Links
PubMed source DOI: 10.1073/pnas.81.7.1966 PMC Full Text View journal details
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