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PMID: 6202368 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A sensitive low artifact TMB procedure for the demonstration of WGA-HRP in the CNS.

Brain research ·Vol. 298 ·No. 2 ·1984-04-30 ·Pages 235-41

Gibson AR, Hansma DI, Houk JC, Robinson FR

Abstract

A TMB reaction with increased sensitivity and lower artifact can be obtained by dividing the Mesulam reaction into two stages. Use of the modified reaction with HRP conjugated wheat germ agglutinin (WGA-HRP) yields a powerful technique for anterograde and retrograde tracing.

MeSH Terms

Animals Axonal Transport Benzidines/metabolism Brain/anatomy & histology Cats Cerebellar Cortex/anatomy & histology Histological Techniques Horseradish Peroxidase/metabolism Lectins/metabolism Nerve Fibers/ultrastructure Olivary Nucleus/anatomy & histology Peroxidases/metabolism Pons/anatomy & histology Rats Red Nucleus/anatomy & histology Retina/anatomy & histology Spinal Cord/anatomy & histology Staining and Labeling Visual Pathways/anatomy & histology Wheat Germ Agglutinins

Chemicals

Benzidines Lectins Wheat Germ Agglutinins 3,3',5,5'-tetramethylbenzidine Horseradish Peroxidase Peroxidases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Gibson A R

Hansma D I

Houk J C

Robinson F R

Article Info

Journal

Brain research

Abbr.

Brain Res

ISSN

0006-8993

DOI

10.1016/0006-8993(84)91423-9

Published

1984-04-30

Pages

235-41

Language

English

Region

Netherlands

NLM ID

0045503

Subset

Grants

NINDS NIH HHS · P01-NS17489 · United States

NINDS NIH HHS · R01-NS14703 · United States

External Links

PubMed source DOI: 10.1016/0006-8993(84)91423-9 View journal details

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A sensitive low artifact TMB procedure for the demonstration of WGA-HRP in the CNS.

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