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PMID: 6203099 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Analysis of male sterile mutations in the mouse using haploid stage expressed cDNA probes.

Nucleic acids research ·Vol. 12 ·No. 10 ·1984-05-25 ·Pages 4281-93

Dudley K, Potter J, Lyon MF, Willison KR

Abstract

A differential hybridization screening procedure has identified cDNAs which correspond to RNAs which are expressed in mouse testis and at lower levels in liver and spleen. The sensitivity of this procedure is such that approximately 0.5% of 1.4 X 10(4) cDNA clones are revealed as "testis specific". We have focused on ten cDNA clones which have been used to identify RNAs expressed in the haploid phase of spermatogenesis. Using Northern blots to analyse RNA isolated from the testes of mutant mice (Tfm/Y and Sxr/+) blocked at specific stages in spermatogenesis or RNA from sexually immature mice, 8 clones have been identified which correspond to RNAs expressed uniquely or at much higher levels in meiotic or post meiotic cells.

MeSH Terms
Animals DNA/metabolism Haploidy Infertility, Male/genetics Male Mice Mice, Inbred Strains Mutation Nucleic Acid Hybridization Poly A/genetics RNA/genetics RNA, Messenger Spermatogenesis Testis/physiopathology
Chemicals
RNA, Messenger Poly A RNA DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dudley K
Potter J
Lyon M F
Willison K R
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27 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.10.4281
Published
1984-05-25
Pages
4281-93
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318831
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/12.10.4281 PMC Full Text View journal details
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