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PMID: 6206076 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A tumor promoter induces rapid and coordinated reorganization of actin and vinculin in cultured cells.

The Journal of cell biology ·Vol. 99 ·No. 3 ·1984-09-00 ·Pages 1045-59

Schliwa M, Nakamura T, Porter KR, Euteneuer U

Abstract

Treatment of epithelial African green monkey kidney (BSC-1) cells with the potent tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) induces a rapid and reversible redistribution of actin and vinculin that is detectable after only 2 min of treatment. Within 20-40 min, stress fibers disappear, while at the same time large actin-containing ribbons resembling ruffles develop both at the cell periphery and in more central regions. Vinculin is associated with these actin ribbons or bands in a punctate or patchy staining pattern. Adhesion to the substratum is changed from predominantly focal contacts associated with stress fiber ends in untreated cells to broad zones of close contact after TPA treatment. High voltage electron microscopic observations disclose the ribbons to consist of highly cross-linked actin filament networks. Thus, association of vinculin with filament networks, rather than (the ends of) filament bundles, is demonstrated. The integrity of microtubules and vimentin filaments is not affected by TPA treatment, but their distribution is altered to conform with the highly distorted cell shape. The response to TPA is neither prevented nor modified by nocodazole-induced depolymerization or taxol-induced stabilization of microtubules. An intact intermediate filament network seems not required either since colcemid-induced collapse of vimentin filaments towards the nucleus does not affect the cell's response to TPA. Rapid redistribution of actin and vinculin also takes place in enucleated cells and in the presence of cycloheximide, but is prevented by dinitrophenol or oligomycin. TPA-induced cytoskeletal alterations are independent of fibronectin expression and not mimicked, modified, or prevented by calmodulin inhibitors or experimentally elevated levels of calcium and cyclic AMP. Thus the morphological response to TPA involves rapid redistribution of actin and vinculin independent of transcription and translation, fluctuations in the levels of calcium or cyclic AMP, or changes in the organization of microtubules, intermediate filaments, and fibronectin.

MeSH Terms
1-Methyl-3-isobutylxanthine/pharmacology Actins/metabolism Animals Benzimidazoles/pharmacology Bucladesine/pharmacology Cell Line Chlorocebus aethiops Cytoskeleton/ultrastructure Fluorescent Antibody Technique Intermediate Filament Proteins/metabolism Kidney Microscopy, Electron Microtubules/ultrastructure Muscle Proteins/metabolism Nocodazole Phorbols/pharmacology Tetradecanoylphorbol Acetate/pharmacology Vimentin Vinculin
Chemicals
Actins Benzimidazoles Intermediate Filament Proteins Muscle Proteins Phorbols Vimentin Vinculin Bucladesine Tetradecanoylphorbol Acetate Nocodazole 1-Methyl-3-isobutylxanthine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schliwa M
Nakamura T
Porter K R
Euteneuer U
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63 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.99.3.1045
Published
1984-09-00
Pages
1045-59
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113420
Subset
IM
Grants
NCRR NIH HHS · RR 00592 · United States
External Links
PubMed source DOI: 10.1083/jcb.99.3.1045 PMC Full Text View journal details
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