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PMID: 6206100 Published · ppublish English Journal Article

Monoclonal antibody to cytokeratin for use in routine histopathology.

Journal of clinical pathology ·Vol. 37 ·No. 9 ·1984-09-00 ·Pages 975-83

Makin CA, Bobrow LG, Bodmer WF

Abstract

CAM 5.2 is a murine monoclonal antibody, raised against the colon carcinoma cell line HT29, which recognises lower molecular weight intracellular cytokeratin proteins within secretory epithelia. Extensive indirect immunohistochemical studies have confirmed that this antibody stains formalin fixed (and freshly frozen) normal and malignant human tissue in a consistent manner. Reliable staining of conventionally processed pathological tissues provides more accurate identification and staging of human malignant epithelial diseases.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Antibodies, Neoplasm/immunology Cell Line Colonic Neoplasms/immunology Fixatives Fluorescent Antibody Technique Humans Hybridomas/immunology Immunoenzyme Techniques Immunoglobulin G/immunology Keratins/analysis,immunology Mice Mice, Inbred BALB C Staining and Labeling
Chemicals
Antibodies, Monoclonal Antibodies, Neoplasm Fixatives Immunoglobulin G Keratins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Makin C A
Bobrow L G
Bodmer W F
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21 references, click to expand
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Article Info
Journal
Journal of clinical pathology
Abbr.
J Clin Pathol
ISSN
0021-9746
DOI
10.1136/jcp.37.9.975
Published
1984-09-00
Pages
975-83
Language
English
Region
England
NLM ID
0376601
PMCID
PMC498911
Subset
IM
External Links
PubMed source DOI: 10.1136/jcp.37.9.975 PMC Full Text View journal details
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