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PMID: 6206566 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Lariat RNA's as intermediates and products in the splicing of messenger RNA precursors.

Science (New York, N.Y.) ·Vol. 225 ·No. 4665 ·1984-08-31 ·Pages 898-903

Padgett RA, Konarska MM, Grabowski PJ, Hardy SF, Sharp PA

Abstract

The splicing of messenger RNA precursors in vitro proceeds through an intermediate that has the 5' end of the intervening sequence joined to a site near the 3' splice site. This lariat structure, which has been characterized for an adenovirus 2 major late transcript, has a branch point, with 2'-5' and 3'-5' phosphodiester bonds emanating from a single adenosine residue. The excised intervening sequence retains the branch site and terminates in a guanosine residue with a 3' hydroxyl group. The phosphate group at the splice junction between the two exons originates from the 3' splice site at the precursor.

MeSH Terms
Adenoviruses, Human/metabolism Base Sequence Chemical Phenomena Chemistry Nucleic Acid Conformation Nucleic Acid Precursors/analysis,metabolism Oligoribonucleotides/metabolism Phosphates/metabolism RNA/analysis,metabolism RNA Precursors RNA Splicing RNA, Messenger/analysis,metabolism RNA, Viral/analysis,metabolism
Chemicals
Nucleic Acid Precursors Oligoribonucleotides Phosphates RNA Precursors RNA, Messenger RNA, Viral RNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Padgett R A
Konarska M M
Grabowski P J
Hardy S F
Sharp P A
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
DOI
10.1126/science.6206566
Published
1984-08-31
Pages
898-903
Language
English
Region
United States
NLM ID
0404511
Subset
IM
Grants
NCI NIH HHS · P01-CA14051 · United States
NCI NIH HHS · P01-CA26717 · United States
NIGMS NIH HHS · R01-GM32467 · United States
Databases
GENBANK
J01917, J01918, J01919, J01920, J01921, J01922, J01923, J01924, J01925, J01926, J01927, J01928, J01929, J01930, J01931, J01932, J01933, J01934, J01935, J01936, J01937, J01938, J01939, J01940, J01941, J01942, J01943, J01944, J01945, J01946
External Links
PubMed source DOI: 10.1126/science.6206566 View journal details
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