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PMID: 6207110 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Unique and common protective epitopes among different serotypes of group A streptococcal M proteins defined with hybridoma antibodies.

Infection and immunity ·Vol. 46 ·No. 1 ·1984-10-00 ·Pages 267-9

Dale JB, Beachey EH

Abstract

A set of four monoclonal antibodies was produced against a highly purified pepsin extract of type 5 streptococcal M protein. Three of the four antibodies cross-reacted with purified M proteins from heterologous serotypes and opsonized the respective heterologous organisms. Our studies suggest that monoclonal antibodies may be useful in identifying subpeptides of various M proteins containing common, protective epitopes that are capable of evoking antibodies that would protect against several different potentially "rheumatogenic" serotypes.

MeSH Terms
Antibodies, Monoclonal/immunology Antigens, Bacterial/immunology Bacterial Outer Membrane Proteins Bacterial Proteins/immunology Carrier Proteins Epitopes Peptide Fragments/immunology Phagocytosis Streptococcus pyogenes/immunology
Chemicals
Antibodies, Monoclonal Antigens, Bacterial Bacterial Outer Membrane Proteins Bacterial Proteins Carrier Proteins Epitopes Peptide Fragments streptococcal M protein
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dale J B
Beachey E H
References (16)
16 references, click to expand
  1. Protective antigenic determinant of streptococcal M protein shared with sarcolemmal membrane protein of human heart.
    J Exp Med. 1982 Oct 1;156(4):1165-76 PMID: 6185606
  2. Epitope-specific protective immunogenicity of chemically synthesized 13-, 18-, and 23-residue peptide fragments of streptococcal M protein.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):2203-7 PMID: 6425829
  3. ANTIGENIC PROPERTIES OF THE TYPE-SPECIFIC SUBSTANCE DERIVED FROM GROUP A HEMOLYTIC STREPTOCOCCI.
    J Exp Med. 1939 Feb 28;69(3):425-45 PMID: 19870855
  4. Repeating covalent structure and protective immunogenicity of native and synthetic polypeptide fragments of type 24 streptococcal M protein. Mapping of protective and nonprotective epitopes with monoclonal antibodies.
    J Biol Chem. 1983 Nov 10;258(21):13250-7 PMID: 6415061
  5. Streptococcal M protein extracted by nonionic detergent. III. Correlation between immunological cross-reactions and structural similarities with implications for antiphagocytosis.
    J Exp Med. 1978 Jun 1;147(6):1771-8 PMID: 355596
  6. Type-specific protective immunity evoked by synthetic peptide of Streptococcus pyogenes M protein.
    Nature. 1981 Jul 30;292(5822):457-9 PMID: 6166868
  7. Hybridoma antibodies against protective and nonprotective antigenic determinants of a structurally defined polypeptide fragment of streptococcal M protein.
    J Exp Med. 1982 Apr 1;155(4):1010-8 PMID: 6174666
  8. Primary structure of protective antigens of type 24 streptococcal M protein.
    J Biol Chem. 1980 Jul 10;255(13):6284-9 PMID: 6156158
  9. Purification and properties of M protein extracted from group A streptococci with pepsin: covalent structure of the amino terminal region of type 24 M antigen.
    J Exp Med. 1977 Jun 1;145(6):1469-83 PMID: 325168
  10. Primary structure of streptococcal Pep M5 protein: Absence of extensive sequence repeats.
    Proc Natl Acad Sci U S A. 1983 Sep;80(18):5475-9 PMID: 16593365
  11. Isotypes of mouse IgG--I. Evidence for 'non-complement-fixing' IgG1 antibodies and characterization of their capacity to interfere with IgG2 sensitization of target red blood cells for lysis by complement.
    Mol Immunol. 1980 Jun;17(6):699-710 PMID: 7432348
  12. Streptococcal M protein extracted by nonionic detergent. II. Analysis of the antibody response to the multiple antigenic determinants of the M-protein molecule.
    J Exp Med. 1977 Oct 1;146(4):1108-23 PMID: 70502
  13. Antiadhesive properties of a quaternary structure-specific hybridoma antibody against type 1 fimbriae of Escherichia coli.
    J Exp Med. 1983 Oct 1;158(4):1114-28 PMID: 6194242
  14. Heterogeneity of type-specific and cross-reactive antigenic determinants within a single M protein of group A streptococci.
    J Exp Med. 1980 May 1;151(5):1026-38 PMID: 6154764
  15. Type-specific immunogenicity of a chemically synthesized peptide fragment of type 5 streptococcal M protein.
    J Exp Med. 1983 Nov 1;158(5):1727-32 PMID: 6195292
  16. Primary structural similarities between types 5 and 24 M proteins of Streptococcus pyogenes.
    Biochem Biophys Res Commun. 1980 Jan 29;92(2):546-53 PMID: 6986870
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.46.1.267-269.1984
Published
1984-10-00
Pages
267-9
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC261470
Subset
IM
Grants
NIAID NIH HHS · AI-10085 · United States
NIAID NIH HHS · AI-13550 · United States
External Links
PubMed source DOI: 10.1128/iai.46.1.267-269.1984 PMC Full Text View journal details
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