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PMID: 6207482 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Developmental regulation of albumin and alpha-fetoprotein gene expression in the rat.

Nucleic acids research ·Vol. 12 ·No. 17 ·1984-09-11 ·Pages 6751-62

Muglia L, Locker J

Abstract

Albumin and AFP comprise a developmentally regulated gene family expressed predominantly in liver and yolk sac. In this study, we have analyzed the developmental changes in the levels of transcripts in liver and yolk sac, and the methylation of the genes in yolk sac. We have previously analyzed the gene methylation in developing liver and non-expressing tissues (Kunnath and Locker, 1983, EMBO J., 2, 317-324). From these analyses we can distinguish 3 active modes of coordinated expression. The adult liver synthesizes albumin, the yolk sac synthesizes AFP, and the fetal liver synthesizes both. In the yolk sac, the relatively inactive albumin gene is hypermethylated, while the active AFP gene is undermethylated. Our data suggest that the genes are activated simultaneously but that subsequent enhancement is specific for tissue and developmental stage.

MeSH Terms
Amniotic Fluid/analysis Animals Blood Proteins/analysis DNA/isolation & purification Female Genes Liver/metabolism Methylation Nucleic Acid Hybridization Pregnancy Proteins/analysis RNA, Messenger/genetics,isolation & purification Rats Rats, Inbred Strains Serum Albumin/genetics Transcription, Genetic Yolk Sac/metabolism alpha-Fetoproteins/genetics
Chemicals
Blood Proteins Proteins RNA, Messenger Serum Albumin alpha-Fetoproteins DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Muglia L
Locker J
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23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.17.6751
Published
1984-09-11
Pages
6751-62
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320114
Subset
IM
Grants
NIGMS NIH HHS · GM07281 · United States
NIGMS NIH HHS · GM27795 · United States
External Links
PubMed source DOI: 10.1093/nar/12.17.6751 PMC Full Text View journal details
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