The high mobility group proteins HMG 14 and 17, do not prevent the formation of chromatin higher order structure.

Nucleic acids research ·Vol. 10 ·No. 6 ·1982-03-25 ·Pages 2007-16

McGhee JD, Rau DC, Felsenfeld G

Abstract

The high mobility group proteins, HMG 14 and 17, have been associated with the chromatin of active genes (refs 1-8), although how they function is not known. We use sedimentation and electric dichroism to investigate the effect of HMG 14 and 17 on the condensation of chicken erythrocyte chromatin into higher order structure. We find no evidence that excess HMG 14 and 17 induce an extended configuration, either in bulk chromatin or in the chromatin of the chicken beta-globulin gene.

MeSH Terms

Animals Chick Embryo Chickens Chromatin/physiology Chromosomal Proteins, Non-Histone/genetics DNA/genetics Erythrocytes/physiology Genes Globins/genetics High Mobility Group Proteins Magnesium/pharmacology Nucleic Acid Conformation Protein Conformation

Chemicals

Chromatin Chromosomal Proteins, Non-Histone High Mobility Group Proteins Globins DNA Magnesium

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

McGhee J D

Rau D C

Felsenfeld G

References (13)

13 references, click to expand

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Involvement of histone H1 in the organization of the nucleosome and of the salt-dependent superstructures of chromatin.
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Interaction of HMG 14 and 17 with actively transcribed genes.
Cell. 1980 Jan;19(1):289-301 PMID: 6244103
Subunit structures of different electrophoretic forms of nucleosomes.
J Biol Chem. 1980 Apr 25;255(8):3673-84 PMID: 7364765
Reconstitution of a deoxyribonuclease I-sensitive structure on active genes.
Proc Natl Acad Sci U S A. 1980 Apr;77(4):1787-90 PMID: 6929520
Orientation of the nucleosome within the higher order structure of chromatin.
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Nucleosome cores have two specific binding sites for nonhistone chromosomal proteins HMG 14 and HMG 17.
Science. 1980 Sep 26;209(4464):1534-6 PMID: 7433974
The interaction of high mobility proteins HMG14 and 17 with nucleosomes.
Nucleic Acids Res. 1980 Sep 11;8(17):3757-78 PMID: 6449690
Isolation of actively transcribed nucleosomes using immobilized HMG 14 and 17 and an analysis of alpha-globin chromatin.
Cell. 1981 Feb;23(2):391-400 PMID: 6258801
A 200 base pair region at the 5' end of the chicken adult beta-globin gene is accessible to nuclease digestion.
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An improved method for the purification of staphylococcal nuclease.
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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/10.6.2007

Published

1982-03-25

Pages

2007-16

Language

English

Region

England

NLM ID

0411011

PMCID

PMC320586

Subset

External Links

PubMed source DOI: 10.1093/nar/10.6.2007 PMC Full Text View journal details

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The high mobility group proteins HMG 14 and 17, do not prevent the formation of chromatin higher order structure.

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