Home LiteratureArticle Details
PMID: 6217195 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Distribution and specificity of mutations induced by neocarzinostatin in the lacI gene of Escherichia coli.

Journal of bacteriology ·Vol. 153 ·No. 1 ·1983-01-00 ·Pages 379-83

Foster PL, Eisenstadt E

Abstract

Although neocarzinostatin (NCS) attacks DNA almost exclusively at adenine and thymine residues in vitro, exposure of Escherichia coli to this antitumor drug resulted in a high frequency of mutations at guanine:cytosine base pairs in the lacI gene. Thus, NCS-induced base substitution mutations do not appear to result from the major DNA lesions that have been biochemically characterized. The overall distribution of nonsense mutations produced by NCS was distinctly nonrandom, consisting in part of a few "hotspots" and a large number of "coldspots." The existence of these coldspots implies that untargeted mutagenesis does not make a significant contribution to the mutations induced by this SOS-dependent mutagen.

MeSH Terms
Antibiotics, Antineoplastic/pharmacology DNA, Bacterial/metabolism Escherichia coli/drug effects,genetics Lac Operon Mutation Zinostatin/pharmacology
Chemicals
Antibiotics, Antineoplastic DNA, Bacterial Zinostatin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Foster P L
Eisenstadt E
References (26)
26 references, click to expand
  1. Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli.
    Bacteriol Rev. 1976 Dec;40(4):869-907 PMID: 795416
  2. Characterization of DNA strand breakage in vitro by the antitumor protein neocarzinostatin.
    Biochemistry. 1977 Feb 8;16(3):486-93 PMID: 189801
  3. Genetic studies of the lac repressor. II. Fine structure deletion map of the lacI gene, and its correlation with the physical map.
    J Mol Biol. 1977 Jan 15;109(2):303-26 PMID: 839544
  4. The relationship between DNA strand-scission and DNA synthesis inhibition in HeLa cells treated with neocarzinostatin.
    Biochim Biophys Acta. 1977 Mar 18;475(2):281-93 PMID: 139166
  5. Effect of DNA Repair systems on antibacterial and mutagenic activity of an antitumor protein, neocarzinostatin.
    Mutat Res. 1977 Apr;48(2):195-203 PMID: 141617
  6. Gaps in DNA induced by neocarzinostatin bear 3'- and 5'-phosphoryl termini.
    Biochemistry. 1978 Feb 21;17(4):729-34 PMID: 146515
  7. Cytological studies on the effect of neocarzinostatin on HeLa cells.
    J Antibiot (Tokyo). 1966 Mar;19(2):69-74 PMID: 5952894
  8. An improved bacterial test system for the detection and classification of mutagens and carcinogens.
    Proc Natl Acad Sci U S A. 1973 Mar;70(3):782-6 PMID: 4577135
  9. Neocarzinostatin-induced breakdown of deoxyribonucleic acid in HeLa-S3 cells.
    J Antibiot (Tokyo). 1975 Feb;28(2):143-8 PMID: 1112766
  10. Mechanism of DNA degradation induced by neocarzinostatin in Bacillus subtilis.
    J Antibiot (Tokyo). 1975 Mar;28(3):229-36 PMID: 123897
  11. In vitro release of thymine from DNA by neocarzinostatin.
    Biochem Biophys Res Commun. 1976 Jan 12;68(1):256-61 PMID: 2169
  12. Genetic studies of the lac repressor. III. Additional correlation of mutational sites with specific amino acid residues.
    J Mol Biol. 1977 Dec 15;117(3):525-67 PMID: 609095
  13. Genetic studies of the lac repressor. IV. Mutagenic specificity in the lacI gene of Escherichia coli.
    J Mol Biol. 1977 Dec 15;117(3):577-606 PMID: 416218
  14. Nucleotide specificity in DNA scission by neocarzinostatin.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3603-7 PMID: 151278
  15. Sequence specific cleavage of DNA by the antitumor antibiotics neocarzinostatin and bleomycin.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3608-12 PMID: 80799
  16. gamma-Ray induced mutational spectrum in the lacI gene of Escherichia coli: comparison of induced and spontaneous spectra at the molecular level.
    Mutat Res. 1980 Jan;69(1):1-12 PMID: 6987490
  17. Roles of chromophore and apo-protein in neocarzinostatin action.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):1970-4 PMID: 6445563
  18. Effect of nonprotein chromophore removal on neocarzinostatin action.
    Biochemistry. 1980 Apr 29;19(9):1767-73 PMID: 6445749
  19. Neocarzinostatin: chemical characterization and partial structure of the non-protein chromophore.
    Biochem Biophys Res Commun. 1980 Aug 14;95(3):1351-6 PMID: 6448053
  20. Mutagenesis by neocarzinostatin in Escherichia coli and Salmonella typhimurium: requirement for umuC+ or plasmid pKM101.
    J Bacteriol. 1980 Nov;144(2):656-60 PMID: 6448839
  21. Deoxyribonucleic acid sugar damage in the action of neocarzinostatin.
    Biochemistry. 1980 Dec 9;19(25):5890-8 PMID: 6450615
  22. Strand scission of deoxyribonucleic acid by neocarzinostatin, auromomycin, and bleomycin: studies on base release and nucleotide sequence specificity.
    Biochemistry. 1981 Dec 22;20(26):7599-606 PMID: 6173064
  23. Covalent adducts of DNA and the nonprotein chromophore of neocarzinostatin contain a modified deoxyribose.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):369-73 PMID: 6210907
  24. Carcinogenic epoxides of benzo[a]pyrene and cyclopenta[cd]pyrene induce base substitutions via specific transversions.
    Proc Natl Acad Sci U S A. 1982 Mar;79(6):1945-9 PMID: 7043469
  25. Random components in mutagenesis.
    Nature. 1982 Sep 23;299(5881):365-7 PMID: 6213867
  26. Acetylornithinase of Escherichia coli: partial purification and some properties.
    J Biol Chem. 1956 Jan;218(1):97-106 PMID: 13278318
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.153.1.379-383.1983
Published
1983-01-00
Pages
379-83
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217383
Subset
IM
Grants
NCI NIH HHS · CA26135 · United States
NIEHS NIH HHS · ES02021 · United States
External Links
PubMed source DOI: 10.1128/jb.153.1.379-383.1983 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2