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PMID: 6217813 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Large-scale isolation of human erythrocyte Ca2+-transport ATPase.

The Biochemical journal ·Vol. 207 ·No. 1 ·1982-10-01 ·Pages 155-9

Gietzen K, Kolandt J

Abstract

A rapid procedure for preparing large quantities of purified erythrocyte Ca2+-transport ATPase is presented. The method involves: (1) fast preparation of calmodulin-deficient, essentially haemoglobin-free, erythrocyte membranes by molecular filtration using Pellicon filters; (2) solubilization of membrane proteins by deoxycholate; and (3) a batch procedure using calmodulin-Sepharose 4B gel for purification of Ca2+-transport ATPase.

MeSH Terms

Calcium-Transporting ATPases/blood,isolation & purification Cell Fractionation Centrifugation Chromatography, Gel Erythrocyte Membrane/enzymology Erythrocytes/enzymology Humans Kinetics Methods Ultrafiltration

Chemicals

Calcium-Transporting ATPases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Gietzen K

Kolandt J

References (14)

14 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2070155

Published

1982-10-01

Pages

155-9

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1153836

Subset

External Links

PubMed source DOI: 10.1042/bj2070155 PMC Full Text View journal details

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Large-scale isolation of human erythrocyte Ca2+-transport ATPase.

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