Home LiteratureArticle Details
PMID: 6221022 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identity and Origin of the ATPase activity associated with neuronal microtubules. I. The ATPase activity is associated with membrane vesicles.

The Journal of cell biology ·Vol. 96 ·No. 5 ·1983-05-00 ·Pages 1298-305

Murphy DB, Hiebsch RR, Wallis KT

Abstract

Microtubule protein purified from brain tissue by cycles of in vitro assembly-disassembly contains ATPase activity that has been postulated to be associated with microtubule-associated proteins (MAPs) and therefore significant for studies of microtubule-dependent motility. In this paper we demonstrate that greater than 90% of the ATPase activity is particulate in nature and may be derived from contaminating membrane vesicles. We also show that the MAPs (MAP-1, MAP-2, and tau factors) and other high molecular weight polypeptides do not contain significant amounts of ATPase activity. These findings do not support the concept of "brain dynein" or of MAPs with ATPase activity.

MeSH Terms
Adenosine Triphosphatases/metabolism Animals Centrifugation, Density Gradient Chromatography, Gel Dyneins/analysis Male Microscopy, Electron Microtubule-Associated Proteins Microtubules/enzymology Neurons/enzymology Proteins/metabolism Swine
Chemicals
Microtubule-Associated Proteins Proteins Adenosine Triphosphatases Dyneins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Murphy D B
Hiebsch R R
Wallis K T
References (34)
34 references, click to expand
  1. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
    Biochemistry. 1971 Jun 22;10(13):2606-17 PMID: 4326772
  2. Interaction of microtubule proteins with phospholipid vesicles.
    J Cell Biol. 1979 Jun;81(3):665-71 PMID: 457778
  3. Adenosine triphosphatase activity of bovine brain microtubule protein.
    J Biol Chem. 1980 Jan 25;255(2):486-91 PMID: 6444297
  4. ATP hydrolase activity associated with microtubules reassembled from bovine splenic nerve--a cautionary tale.
    J Neurochem. 1976 Dec;27(6):1465-71 PMID: 187733
  5. Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels.
    Biochem Biophys Res Commun. 1967 Sep 7;28(5):815-20 PMID: 4861258
  6. Some characteristics of ATPase activity in a brain microtubule protein preparation.
    J Neurochem. 1979 Dec;33(6):1249-58 PMID: 162206
  7. A dynein-like protein associated with neurotubules.
    FEBS Lett. 1974 Apr 1;40(2):281-6 PMID: 4277331
  8. Studies on the adenosine triphosphatase activity of 14 S and 30 S dynein from cilia of Tetrahymena.
    J Biol Chem. 1966 Dec 10;241(23):5590-6 PMID: 4224623
  9. Dynein binds to and crossbridges cytoplasmic microtubules.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5759-63 PMID: 160555
  10. Vanadate is a potent (Na,K)-ATPase inhibitor found in ATP derived from muscle.
    J Biol Chem. 1977 Nov 10;252(21):7421-3 PMID: 144127
  11. Selective release of content from microsomal vesicles without membrane disassembly. I. Permeability changes induced by low detergent concentrations.
    J Cell Biol. 1973 Aug;58(2):436-62 PMID: 4729506
  12. Association between endocrine pancreatic secretory granules and in-vitro-assembled microtubules is dependent upon microtubule-associated proteins.
    J Cell Biol. 1982 Apr;93(1):164-74 PMID: 7040413
  13. Acanthamoeba myosin. I. Isolation from Acanthamoeba castellanii of an enzyme similar to muscle myosin.
    J Biol Chem. 1973 Jul 10;248(13):4682-90 PMID: 4268863
  14. Decoration of spindle microtubules with Dynein: evidence for uniform polarity.
    J Cell Biol. 1981 May;89(2):373-8 PMID: 6454693
  15. A new ATPase in cytoplasmic microtubule preparations.
    FEBS Lett. 1978 Apr 15;88(2):197-200 PMID: 148371
  16. Chemical dissection of cilia.
    Arch Biol (Liege). 1965;76(2):317-52 PMID: 5323574
  17. Ultrastructural localization of the high molecular weight proteins associated with in vitro-assembled brain microtubules.
    J Cell Biol. 1975 Apr;65(1):237-41 PMID: 1127013
  18. Azide inhibition of mitochondrial ATPase.
    Biochem Biophys Res Commun. 1968 Jan 11;30(1):83-8 PMID: 4230013
  19. An ATPase activity associated with brain microtubules.
    Arch Biochem Biophys. 1979 Nov;198(1):296-303 PMID: 159665
  20. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  21. The influence of some cations on an adenosine triphosphatase from peripheral nerves.
    Biochim Biophys Acta. 1957 Feb;23(2):394-401 PMID: 13412736
  22. Purification and properties of an adenosine triphosphatase from sarcoplasmic reticulum.
    J Biol Chem. 1970 Sep 10;245(17):4508-18 PMID: 4250726
  23. A dynein-like protein from brain.
    FEBS Lett. 1974 Apr 1;40(2):274-80 PMID: 4277332
  24. Turnover of constituents of the endoplasmic reticulum membranes of rat hepatocytes.
    J Biol Chem. 1967 May 25;242(10):2389-96 PMID: 4381697
  25. Identity and origin of the ATPase activity associated with neuronal microtubules. II. Identification of a 50,000-dalton polypeptide with ATPase activity similar to F-1 ATPase from mitochondria.
    J Cell Biol. 1983 May;96(5):1306-15 PMID: 6221023
  26. A simple method for the isolation and purification of total lipides from animal tissues.
    J Biol Chem. 1957 May;226(1):497-509 PMID: 13428781
  27. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  28. Dynein-like Mg2+-ATPase in mitotic spindles isolated from sea urchin embryos (Strongylocentrotus droebachiensis).
    J Cell Biol. 1980 Sep;86(3):738-45 PMID: 6447705
  29. Binding of microtubules to pituitary secretory granules and secretory granule membranes.
    J Cell Biol. 1977 Feb;72(2):380-9 PMID: 833201
  30. The use of oligomycin as an inhibitor of oxidative phosphorylation.
    J Biochem. 1961 Jun;49:493-501 PMID: 13716716
  31. Identity and polymerization-stimulatory activity of the nontubulin proteins associated with microtubules.
    Biochemistry. 1977 Jun 14;16(12):2598-605 PMID: 889779
  32. Association of high-molecular-weight proteins with microtubules and their role in microtubule assembly in vitro.
    Proc Natl Acad Sci U S A. 1975 Jul;72(7):2696-700 PMID: 1058484
  33. The presence of an adenosine-5'-triphosphatase dependent on 6S tubulin and calcium ions in rat brain microtubules.
    J Biochem. 1979 Aug;86(2):587-90 PMID: 158016
  34. Purification of tubulin and associated high molecular weight proteins from porcine brain and characterization of microtubule assembly in vitro.
    Ann N Y Acad Sci. 1975 Jun 30;253:107-32 PMID: 1056738
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.96.5.1298
Published
1983-05-00
Pages
1298-305
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2112664
Subset
IM
Grants
NCI NIH HHS · CA 00645 · United States
NIGMS NIH HHS · GM 26155 · United States
External Links
PubMed source DOI: 10.1083/jcb.96.5.1298 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2